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Item 1 of 4
EphB2 Antibody (C-term)
Catalog Number: orb1929080
| Catalog Number | orb1929080 |
|---|---|
| Category | Antibodies |
| Description | Purified Rabbit Polyclonal Antibody (Pab) |
| Target | This EphB2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1021-1050 amino acids from the C-terminal region of human EphB2. |
| Clonality | Polyclonal |
| Species/Host | Rabbit |
| Isotype | Rabbit IgG |
| Conjugation | Unconjugated |
| Reactivity | Human |
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
| UniProt ID | P29323 |
| MW | 117493 Da |
| Tested applications | IF, IHC-P, WB |
| Dilution range | IF: 1:10~50, WB: 1:1000, IHC-P: 1:10~50, IHC-P: 1:10~50 |
| Antibody Type | Primary Antibody |
| Clone Number | RB01652 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
| Alternative names | Ephrin type-B receptor 2, Developmentally-regulate Read more... |
| Note | For research use only |
| NCBI | NP_059145.2, NP_004433.2 |
Western blot analysis of anti-EphB2 C-term Pab in NCI-H460 cell lysate. EphB2 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
Confocal immunofluorescent analysis of EphB2 Antibody (C-term) with A375 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).
Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with EphB2 antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Formalin-fixed and paraffin-embedded human breast carcinoma reacted with EphB2 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
