EGFR Mouse Monoclonal Antibody

SKU: orb500711

Description

EGFR Mouse Monoclonal Antibody

Research Area

Cell Biology

Images & Validation

• Tested Applications: KO/KD Validated, WB
• Dilution Range: WB=1:1000-5000
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG
• Clone No.: B3C8
• Immunogen: KLH conjugated synthetic peptide derived from human EGFR
• Target: EGFR
• Molecular Weight: 170-175 kDa
• Purification: Affinity purified by Protein G
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

ERBB; ERBB1; ERRP; HER1; NISBD2; NNCIS; PIG61; mENA; 9030024J15Rik; Errb1; Wa5; wa-2; wa2; ErbB-1; EGFR_HUMAN; EGFR; Proto-oncogene c-ErbB-1; Receptor tyrosine-protein kinase erbB-1; 2.7.10.1; EGFR_MOUSE; epidermal growth factor receptor; epidermal growth factor receptor (avian erythroblastic leukemia viral (v-erb-b) oncogene homolog); erythroblastic leukemia viral (v-erb-b) oncogene homolog (avian); erb-b2 receptor tyrosine kinase 1

Paraformaldehyde-fixed, paraffin embedded (Human brain glioma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (EGFR) Monoclonal Antibody, Unconjugated (orb500711) at 1:400 overnight at 4°C, followed by a conjugated Goat Anti-Mouse IgG antibody for 90 minutes, and DAPI for nuclei staining.

Paraformaldehyde-fixed, paraffin embedded (Human brain glioma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (EGFR) Monoclonal Antibody, Unconjugated (orb500711) at 1:400 overnight at 4°C, followed by a conjugated Goat Anti-Mouse IgG antibody for 90 minutes, and DAPI for nuclei staining.

Sample: HUVEC (Human) Cell Lysate at 30 ug, Primary: Anti-EGFR (orb500711) at 1/500 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 170 kD, Observed band size: 170 kD.

Tissue/Cell: Hela cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (EGFR) monoclonal Antibody, Unconjugated (orb500711) 1:100, 90 minutes at 37°C, followed by a CY3 conjugated Goat Anti-Mouse IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

Quick Database Links

Gene Symbol

EGFR

UniProt

P00533