EGFR Antibody / EGF Receptor

SKU: orb749350

Description

Mouse monoclonal antibody against EGF Receptor conjugated to CF488

Research Area

Cancer, Developmental Biology, Epigenetics, Infectious Diseases

Images & Validation

• Tested Applications: FACS, IF
• Dilution Range: Flow cytometry: 5ul per test per one 10^6 cells in 0.1ml or 5ul per 100ul of whole blood,Immunofluorescence: 1:50-1:100
• Reactivity: Human
• Application Notes:
  Optimal dilution of the EGFR antibody should be determined by the researcher.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG2a, kappa
• Clone No.: GFR450
• Immunogen: Recombinant extracellular domain of human EGFR protein was used as the immunogen for the EGFR antibody.
• Purification: Protein G affinity chromatography
• Conjugation: CF488

Storage & Handling

• Storage: Store the EGFR antibody at 2-8°C, protected from light.
• Buffer/Preservatives: 500 ul at 0.1 mg/ml with 0.1 mg/ml rAlbumin, 0.05% sodium azide
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Left: Immunofluorescent staining of human A431 cells with CF488-conjugated EGFR antibody (clone GFR450, green) and DAPI nuclear stain (blue). Right: isotype control.

Flow cytometry testing of human A431 cells with EGFR antibody (clone GFR450); Black = cells alone, Gray = isotype control, Green = EGFR antibody.

Flow cytometry testing of mouse NIH3T3 cells with EGFR antibody (clone GFR450); Black = cells alone, Gray = isotype control, Green = EGFR antibody. This antibody is not suitable for mouse sample testing.

Quick Database Links

UniProt

P00533