EGF Receptor / EGFR Antibody

• Catalog Number: orb749350
• Category: Antibodies
• Description: Mouse monoclonal antibody against EGF Receptor conjugated to CF488
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: Mouse IgG2a, kappa
• Conjugation: CF488
• Reactivity: Human
• Immunogen: Recombinant extracellular domain of human EGFR protein was used as the immunogen for the EGFR antibody.
• UniProt ID: P00533
• Tested applications: FACS, IF
• Dilution range: Flow cytometry: 5ul per test per one million cells in 0.1ml or 5ul per 100ul of whole blood,Immunofluorescence: 1:50-1:100
• Application notes: Optimal dilution of the EGFR antibody should be determined by the researcher.
• Antibody Type: Primary Antibody
• Clone Number: GFR450
• Formula: 500 ul at 0.1 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Storage: Store the EGFR antibody at 2-8°C, protected from light.
• Research Area: Cancer, Developmental Biology, Epigenetics, Infect
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Left: Immunofluorescent staining of human A431 cells with CF488-conjugated EGFR antibody (clone GFR450, green) and DAPI nuclear stain (blue). Right: isotype control.

Flow cytometry testing of human A431 cells with EGFR antibody (clone GFR450); Black = cells alone, Gray = isotype control, Green = EGFR antibody.

Flow cytometry testing of mouse NIH3T3 cells with EGFR antibody (clone GFR450); Black = cells alone, Gray = isotype control, Green = EGFR antibody. This antibody is not suitable for mouse sample testing.