EGF Receptor / EGFR Antibody

• Catalog Number: orb639734
• Category: Antibodies
• Description: The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. Multiple alternatively spliced transcript variants that encode different protein isoforms have been found for this gene.
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: Mouse IgG1, kappa
• Conjugation: Unconjugated
• Reactivity: Human
• Immunogen: Recombinant full-length human Epidermal growth factor receptor protein was used as the immunogen for the EGFR antibody.
• UniProt ID: P00533
• Tested applications: IHC-P, WB
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml,Western blot: 1-2ug/ml
• Application notes: Optimal dilution of the antibody should be determined by the researcher.
• Antibody Type: Primary Antibody
• Clone Number: rGFR/1667
• Formula: 0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Research Area: Cancer, Developmental Biology, Epigenetics, Infect
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot testing of human A431 cell lysate with EGFR antibody (clone rGFR/1667). Expected molecular weight: ~134/170 kDa (unmodified/glycosylated).

SDS-PAGE analysis of purified, BSA-free EGFR antibody (clone rGFR/1667) as confirmation of integrity and purity.