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Catalog Number | orb639674 |
---|---|
Category | Antibodies |
Description | This MAb recognizes a protein of 170kDa, identified as EGFR. EGFR is type I receptor tyrosine kinase with sequence homology to erbB-1, -2, -3 -4 or HER-1, -2, -3 -4. It binds to Epidermal Growth Factor (EGF), Transforming Growth Factor-a (TGF-a), Heparin-binding EGF (HB-EGF), amphiregulin, betacellulin and epiregulin. EGFR is overexpressed in tumors of breast, brain, bladder, lung, gastric, head neck, esophagus, cervix, vulva, ovary, and endometrium. It is predominantly present in squamous cell carcinomas. |
Species/Host | Mouse |
Clonality | Monoclonal |
Clone Number | SPM341 |
Tested applications | IHC-P, WB |
Reactivity | Human |
Isotype | Mouse IgG2b, kappa |
Immunogen | A human recombinant partial protein (amino acids 344-492) was used as the immunogen for the EGFR antibody. |
Dilution range | Immunohistochemistry (FFPE): 1-2ug/ml,Western blot: 1-2ug/ml |
Purity | Protein G affinity chromatography |
Conjugation | Unconjugated |
Formula | 0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide |
Hazard Information | This EGFR antibody is available for research use only. |
UniProt ID | P00533 |
Storage | Store the EGFR antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide). |
Buffer/Preservatives | 0.2 mg/ml with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide |
Note | For research use only |
Application notes | Optimal dilution of the antibody should be determined by the researcher. |
Expiration Date | 12 months from date of receipt. |
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