EGFR Antibody / EGF Receptor

SKU: orb2636438

Description

EGFR (Epidermal growth factor receptor, HER1, ErbB1) is encoded by the EGFR gene located on chromosome 7 in humans. EGFR belongs to the HER/ERbB family of proteins that includes three other receptor tyrosine kinases, ERbB2, ERbB3, ERbB4. EGFR is a transmembrane receptor and binding of its cognate ligands such as EGF (Epidermal Growth Factor) and TGF alpha (Transforming Growth Factor alpha) to the extracellular domain leads to EGFR dimerization followed by autophosphorylation of the tyrosine residues in the cytoplasmic domain.

Images & Validation

• Tested Applications: ELISA, IF, WB
• Dilution Range: Immunofluorescence: 1-3ug/ml,Western blot: 2-4ug/ml
• Reactivity: Human
• Application Notes:
  Optimal dilution of the EGFR antibody should be determined by the researcher.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG2b, kappa
• Clone No.: 225
• Immunogen: Purified EGF Receptor protein from A431 cells was used as the immunogen for the EGFR antibody.
• Purification: Protein A/G affinity
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin, 0.05% sodium azide
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

SDS-PAGE analysis of purified, BSA-free EGFR antibody (clone 225) as confirmation of integrity and purity.

Quick Database Links

UniProt

P00533