NKX2-5 Antibody

SKU: orb1249612

Description

NKX2-5 Antibody

Images & Validation

• Tested Applications: ELISA, IF, WB
• Reactivity: Human, Mouse
• Predicted Reactivity: Bovine, Canine, Rat
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 40-45kDa band observed in Mouse Heart lysates (calculated MW of 34.2kDa according to NP_032726.1). Preliminary experimants also showed an apprix 45kDa band in Human and Rat Heart kysates. The observed molecular weight corresponds to earlier findings in literature with different antibodies (Shiojima et al, Circ Res. 1996 Nov;79(5):920-9.; PMID: 8888684). Recommended concentration: 0.1-0.5µg/mL. Primary incubation 1 hour at room temperature.Immunofluorescence: Strong expression of the protein seen in the nucleus and cytoplasm of U2OS cells. Recommended concentration: 10µg/mL.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: C-PRAYSDPDPAKDPR
• Target: NKX2-5
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

NKX2-5, NK2 transcription factor related, locus 5 (Drosophila), CHNG5, CSX, CSX1, NKX2.5, NKX2E, NKX4-1, NK2 transcription factor homolog E, NK2 transcription factor related, locus 5, cardiac-specific homeo box, tinman paralog

orb1249612 (0.3 ug/ml) staining of Mouse Heart lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1249612 (3.75 ug/ml) staining of paraffin embedded Human Heart. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. This data is from a previous batch, not on sale.

orb1249612 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing nuclear and cytoplasmic staining.

Quick Database Links

Gene Symbol

NKX2-5

UniProt

P52952

RefSeq (Protein):NP_001159648.1, NP_001159647.1, NP_004378.1