Mouse Nkx2-5 Antibody (Center)

• Catalog Number: orb1926865
• Category: Antibodies
• Description: Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB51144
• Tested applications: WB
• Reactivity: Hamster, Mouse, Rat
• Isotype: Rabbit IgG
• Dilution range: WB: 1:2000, WB: 1:1000, WB: 1:2000
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• MW: 34163 Da
• Target: This Mouse Nkx2-5 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 192-225 amino acids from the Central region of human Mouse Nkx2-5.
• UniProt ID: P42582
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Homeobox protein Nkx-25, Cardiac-specific homeobox
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Anti-Mouse Nkx2-5 Antibody (Center) at 1:2000 dilution + CCRF-CEM whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 34 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-Mouse Nkx2-5 Antibody (Center) at 1:2000 dilution. Lane 1: human heart lysate. Lane 2: mouse heart lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 34 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Western blot analysis of lysates from mouse stomach, rat heart tissue (from left to right), using Mouse Nkx2-5 Antibody (Center). Diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20 ug per lane.