Mouse Nkx2-5 Antibody (Center)

SKU: orb1926865

Description

Mouse Nkx2-5 Antibody (Center)

Images & Validation

• Tested Applications: WB
• Dilution range: WB - 1:2000
• Reactivity: Hamster, Human, Mouse, Rat

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Molecular Weight: 34163 Da

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Disclaimer: For research use only

Alternative Names

Csx, Nkx-2.5, Nkx2e

Anti-Mouse Nkx2-5 Antibody (Center) at 1:2000 dilution + CCRF-CEM whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 34 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-Mouse Nkx2-5 Antibody (Center) at 1:2000 dilution. Lane 1: human heart lysate. Lane 2: mouse heart lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 34 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Western blot analysis of lysates from mouse stomach, rat heart tissue (from left to right), using Mouse Nkx2-5 Antibody (Center). Diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20 ug per lane.

Quick Database Links

UniProt

P42582