CSX1/NKX2-5 Antibody

SKU: orb20262

Description

Goat polyclonal antibody to NKX2-5

Research Area

Cardiovascular Disease

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution Range: ELISA: 1:32000, WB: 0.1-0.5 μg/ml, IHC-P: 10 μg/ml
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Bovine, Canine
• Application Notes:
  WB: Approx. 38kDa band observed in Human, Mouse and Rat Heart lysates (calculated MW of 34.9kDa according to Human NP_004378.1, 34.2kDa according to Mouse NP_032726.1 and 34.3kDa according to Rat NP_446103.2). Recommended concentration: 1-3µg/ml. Primary incu. IHC: Paraffin embedded Human Heart. Recommended concentration: 5-6µg/ml.

Key Properties

• Host: Goat
• Target: CSX1 / NKX2-5
• Protein Sequence: PRAYSDPDPAKDPR
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

NKX2-5; NK2 transcription factor related, locus 5 (Drosophila); CHNG5; CSX; CSX1; NKX2.5; NKX2E; NKX4-1; NK2 transcription factor homolog E; NK2 transcription factor related, locus 5; cardiac-specific homeo box; tinman paralog

1 µg/ml staining of Mouse Heart lysate (35 µg protein in RIPA buffer). Detected by chemiluminescence.

5 µg/ml staining of paraffin embedded Human Heart. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.

Negative Control showing staining of paraffin embedded Human Heart, with no primary antibody.

Quick Database Links

Gene Symbol

CSX1 / NKX2-5