NKX2-5 Rabbit pAb Discontinued

SKU: orb540657

Description

Rabbit polyclonal antibody to NKX2-5

Research Area

Developmental Biology

Images & Validation

• Tested Applications: ELISA, WB
• Dilution Range: WB: 1:500-1:2000, IF: 1:50-1:200
• Reactivity: Human, Mouse, Rat

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-135 of human NKX2-5 (NP_004378.1).
• Molecular Weight: 37kDa
• Purification: Affinity purification
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

NKX2-5, CHNG5, CSX, CSX1, HLHS2, NKX2.5, NKX2E, NKX4-1, VSD3

Western blot analysis of extracts of various cell lines, using NKX2-5 antibody (orb540657) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 60s.

Quick Database Links

UniProt

P52952

Hamid Najafipour 1, Farzaneh Rostamzadeh 2, Seedieh Jafarinejad-Farsangi 3, Zahra Bagheri-Hosseinabadi 4, Elham Jafari 5, Alireza Farsinejad 6, Mohmmad Mehdi Bagheri 7 Human platelet lysate combined with mesenchymal stem cells pretreated with platelet lysate improved cardiac function in rats with myocardial infarction Sci Rep, (2024)

PubMed: 39533052