Celecoxib

SKU: orb1310585

Description

Celecoxib (SC-58635) is a selective COX-2 inhibitor (IC50=40 nM) with demonstrated anti-inflammatory and analgesic properties. It is widely used in research models of osteoarthritis, rheumatoid arthritis, and pain, employing both in vitro enzyme assays and in vivo animal studies to investigate its mechanisms and efficacy.

Research Area

Immunology & Inflammation, Neuroscience, Pharmacology & Drug Discovery

Key Properties

• CAS Number: 169590-42-5
• MW: 381.4
• Purity: >98%
• Formula: C₁₇H₁₄F₃N₃O₂S
• SMILES: C(F)(F)(F)C1=NN(C(=C1)C2=CC=C(C)C=C2)C3=CC=C(S(N)(=O)=O)C=C3
• Target: COX (Cyclooxygenase)
• Solubility: Soluble in DMSO (up to 100 mg/ml) or in Ethanol (up to 100 mg/ml).

Bioactivity

• Target IC50:
  COX-1:15 μM|A2058 cells:63 ± 4 µM|Hone-1 cells:49.68 ± 1.12 μM|HK-1 cells:51.74 ± 3.89 μM|COX-2:40 nM|CNE-2 cells:41.04 ± 1.22 μM
• In Vivo:
  Celecoxib inhibits cell proliferation in nasopharyngeal carcinoma cell lines HNE1 (IC50=32.86 μM) and CNE1-LMP1 (IC50= 61.31 μM). It also selectively inhibits COX-1 (IC50=15 μM) and COX-2 (IC50=40 nM) with effective selectivity.
• In Vitro:
  In rats, Celecoxib at doses ranging from 200 to 600 mg/kg does not induce acute gastrointestinal (GI) toxicity. Furthermore, continuous treatment with Celecoxib at these dosages for over ten days fails to produce GI toxicity. In the adjuvant-induced arthritis model, Celecoxib mitigates chronic inflammation with an effective dose 50 (ED50) of 0.37 mg/kg. Additionally, the compound exhibits analgesic activity in the Hargreaves pain model, with an ED50 of 34.5 mg/kg.
• Cell Research:
  The antiproliferative effect of Celecoxib on NPC cells is assessed using an MTT assay. Cells are seeded into 96-well plates and allowed to attach for 24 hours. The cells are then treated with increasing concentrations of Celecoxib (0 to 75 μM) dissolved in DMSO (final concentration ≤0.1%) and incubated for up to 48 hours. After the incubation, 20 μL of MTT dye (5 mg/mL) are added to each well and cells are incubated at 37 °C for 4 hours. After removing the supernatants, the crystals are dissolved in DMSO and the absorbance is measured at 490 nm. The half-maximal inhibitory concentration (IC50) values and the 95% confidence intervals are calculated using probit regression using SPSS 15.0 software. The experiment is performed in triplicate and repeated at least three times.(Only for Reference)

Storage & Handling

• Storage: RT
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

SC-58635

Compound Identifiers

PubChem CID

2662