CD45RA Antibody (Leukocyte marker)

• Catalog Number: orb385828
• Category: Antibodies
• Description: CD45RA is an isoform of the human leukocyte common antigen (CD45). Human CD45 contains three exons which encode peptide segments designated A, B and C, respectively. The differential splicing of the exons generates at least five isoforms, ABC, AB, BC, B and O. CD45RA is expressed on 40-50% of peripheral CD4+ T-cells, 50% of peripheral CD8+ T-cells, B-cells, and leukemic B-cell lines. T-cells expressing CD45RA are naive or virgin T-cells. T-cells expressing CD45RO are memory T-cells. CD45RA and CD45RO define complementary, predominantly non-overlapping populations of resting peripheral T-cells.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: CDLA45RA-1
• Tested applications: FACS, IF, IHC, WB
• Reactivity: Human
• Isotype: Mouse IgG2a, kappa
• Immunogen: Recombinant human protein was used as immunogen for this anti-CD45RA antibody.
• Antibody Type: Primary Antibody
• Dilution range: Western blot: 1-2ug/ml,Flow cytometry: 0.5-1ug/million cells,Immunofluorescence: 1-2 ug/ml,Immunohistology (FFPE): 0.5-1.0ug/ml for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1)
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This anti-CD45RA antibody is available for research use only.
• UniProt ID: P08575
• Storage: The anti-CD45RA antibody (with azide) can be stored at 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: Titering of the anti-CD45RA antibody may be required for optimal performance.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

IHC testing of FFPE human tonsil and anti-CD45RA antibody (clone CDLA45RA-1). HIER: boil sections in pH6 10 mM citrate buffer for 10-20 minutes, followed by cooling at RT for 20 minutes, prior to staining.

Flow cytometry testing of PFA-fixed human Jurkat cells with CD45RA antibody (clone CDLA45RA-1); Red = isotype control, Blue = CD45RA antibody.

Western blot testing of human spleen lysate with CD45RA antibody (clone CDLA45RA-1). Expected molecular weight: 147-220 kDa depending on glycosylation level.

Immunofluorescence staining of PFA-fixed human Jurkat cells with CD45RA antibody (clone CDLA45RA-1, green) and Reddot nuclear stain (red).