CD45RA Antibody (Leukocyte marker)

• Catalog Number: orb248519
• Category: Antibodies
• Description: This antibody recognizes a protein of 205kDa-220kDa, identified as CD45RA, an isoform of the human leukocyte common antigen (CD45). Human CD45 contains three exons which encode peptide segments designated A, B and C. The differential splicing of the exons generates at least five isoforms, ABC, AB, BC, B and O. This antibody reacts with ABC and BC isoforms. CD45RA is expressed on 40-50% of peripheral CD4+ Tcells, 50% of peripheral CD8+ T-cells, B-cells, and leukemic B-cell lines. T-cells expressing CD45RA are naive or virgin Tcells. T-cells expressing CD45RO are memory Tcells. CD45RA and CD45RO define complementary, predominantly non-overlapping populations of resting peripheral Tcells. This CD45RA antibody is useful in the study of the subpopulation of CD4+ or CD8+ T-cells. It is especially useful in differentiating T-cell lymphomas (CD45RO +ve) from B cell lymphomas (CD45RA +ve).
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: Mouse IgG2a, kappa
• Conjugation: Unconjugated
• Reactivity: Human
• Immunogen: Stimulated human leukocytes were used as the immunogen for this CD45RA antibody.
• Tested applications: FACS, IF, IHC-P, WB
• Dilution range: Flow cytometry: 0.5-1ug/million cells,Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT
• Application notes: The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the CD45RA antibody to be titered up or down for optimal performance.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Antibody Type: Primary Antibody
• Clone Number: 158-4D3
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunofluorescent staining of PFA-fixed human Raji cells with CD45RA antibody (clone 158-4D3, green) and Reddot nuclear stain (red).

IHC staining of FFPE human tonsil with CD45RA antibody (clone 158-4D3). HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.

FACS surface staining of human PBMCs using CD45RA antibody (clone 158-4D3, red), and isotype control (green).

Flow cytometry testing of human Jurkat cells with CD45RA antibody (clone 158-4D3); Red = isotype control, Blue = CD45RA antibody.

Western blot testing of human spleen lysate with CD45RA antibody (clone 158-4D3). Expected molecular weight: 147-220 kDa depending on glycosylation level.

SDS-PAGE analysis of purified, BSA-free CD45RA antibody (clone 158-4D3) as confirmation of integrity and purity.