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Item 1 of 5
Item 1 of 5
Anti-Hu CD45RA Purified
Catalog Number: orb44329
| Catalog Number | orb44329 |
|---|---|
| Category | Antibodies |
| Description | Mouse Monoclonal to CD45RA. |
| Clonality | Monoclonal |
| Clone Number | MEM-56 |
| Tested applications | FC, IHC-P, IP, WB |
| Reactivity | Human |
| Isotype | Mouse IgG2b |
| Immunogen | Human thymocytes and T lymphocytes. |
| Antibody Type | Primary Antibody |
| Concentration | 1 mg/ml |
| Dilution range | Immunohistochemistry (paraffin sections): No pre-treatment of tissue sections is needed. Flow cytometry: Recommended dilution: 1-4 μg/ml. |
| Purity | Purified by protein-A affinity chromatography. |
| Conjugation | Unconjugated |
| Target | CD45RA |
| RRID | AB_10989853 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Buffer/Preservatives | Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
| Alternative names | Anti-CD45RA antibody Read more... |
| Note | For research use only |
| Application notes | Immunohistochemistry (paraffin sections): No pre-treatment of tissue sections is needed. Flow cytometry: Recommended dilution: 1-4 μg/ml. |
| Expiration Date | 12 months from date of receipt. |
Mass cytometry (surface staining) of human peripheral blood with anti-CD45RA (MEM-56) 163Dy. Gated on singlets.
Western blotting analysis of whole cell lysate of HUT-78 human cutaneous T cell lymphoma cell line. Lane 1: immunostaining with anti-human CD45 control antibody; Lane 2: immunostaining with anti-human CD45RA (MEM-56); sample of cell lysate prepared under non-reducing conditions; Lane 3: immunostaining with anti-human CD45RA (MEM-56); sample of cell lysate prepared under reducing conditions.
Separation of human CD45RA positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral blood stained using anti-human CD45RA (MEM-56) purified antibody (concentration in sample 0.3 µg/ml, GAM APC).
Flow cytometry surface staining pattern of human peripheral blood stained using anti-human CD45RA (MEM-56) purified antibody (concentration in sample 0.3 µg/ml, GAM APC).
Immunohistochemistry staining of human tonsil (paraffin-embedded sections) with anti-CD45RA (MEM-56), 5 µg/ml.
