CD45 Antibody (Leukocyte marker)

SKU: orb606479

Description

CD45R, also designated CD45 and PTPRC, has been identified as a transmembrane glycoprotein, broadly expressed among hematopoietic cells. Multiple isoforms of CD45R are distributed throughout the immune system according to cell type. These isoforms arise because of alternative splicing of exons 4, 5, and 6. The corresponding protein domains are characterized by the binding of monoclonal antibodies specific for CD45RA (exon 4), CD45RB (exon 5), CD45RC (exon 6) and CD45RO (exons 4 to 6 spliced out). The variation in these isoforms is localized to the extracellular domain of CD45R, while the intracellular domain is conserved. CD45R functions as a phosphor-tyrosine phosphatase. Antibody to CD45 is useful in differential diagnosis of lymphoid tumors from non-hematopoietic undifferentiated neoplasms.

Images & Validation

• Tested Applications: FACS, IF, IHC-P, WB
• Dilution range: Flow cytometry: 0.5-1ug/million cells,Western blot: 1-2ug/ml,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT
• Reactivity: Human
• Application Notes:
  Due to variation in protocol and secondary antibody used, the recombinant CD45 antibody may need to be titered for optimal performance.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Recombinant
• Isotype: Rabbit IgG, kappa
• Clone No.: PTPRC/1975R
• Immunogen: Recombinant human protein was used as immunogen for this recombinant CD45 antibody.

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Disclaimer: For research use only

IHC testing of recombinant CD45 antibody and FFPE human lymph node (clone PTPRC/1975R). Required HIER: boil tissue sections in 10mM citrate buffer, pH6, for 10-20 min followed by cooling at RT for 20 min.

Flow cytometry testing of PFA-fixed human Raji cells with CD45 antibody (clone PTPRC/1975R); Red = isotype control, Blue = CD45 antibody.

Western blot testing of human spleen lysate with CD45 antibody (clone PTPRC/1975R). Expected molecular weight: 147-220 kDa depending on glycosylation level.

Immunofluorescence staining of human Raji cells with CD43 antibody (clone PTPRC/1975R, green) and Phalloidin (red).

SDS-PAGE analysis of purified, BSA-free recombinant CD45 antibody (clone PTPRC/1975R) as confirmation of integrity and purity.

Quick Database Links

UniProt

P08575