CD28 Antibody

• Catalog Number: orb749882
• Category: Antibodies
• Description: Recognizes a glycoprotein of 44-88kDa, which is identified as CD28. It is the critical T-cell co-stimulatory receptor which provides to the cell the important second activation signal by binding CD80 and CD86 that are expressed by antigen presenting cells. Besides its co-stimulation role, CD28 functions in preventing T-cells from anergic hyporesponsive state or from undergoing premature apoptotic cell death. CD28 is also expressed on human fetal NK cells and some NK cell lines, whereas on murine NK cells the CD28 expression is much broader.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 204.12
• Tested applications: FACS, IF
• Reactivity: Human, Mouse
• Isotype: Mouse IgG2a, kappa
• Immunogen: Stimulated human leukocytes were used as the immunogen for the CD28 antibody.
• Dilution range: Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml Functional studies (order BSA/sodium azide-free format)
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This CD28 antibody is available for research use only.
• UniProt ID: P10747
• Storage: Store the CD28 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the CD28 antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.