Goat anti-CD80 Antibody

• Catalog Number: orb20356
• Category: Antibodies
• Description: Goat polyclonal to CD80
• Target: CD80
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: HLRVNQTFNWNTTK
• RRID: AB_10748963
• MW: 33
• Tested applications: ELISA, FC, IHC, WB
• Dilution range: ELISA: 1:4000, WB: 0.3-1 μg/ml
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:4000.WB: Approx 60kDa band observed in Human Lymph Node and Human Tonsil lysates (calculated MW of 33.0kDa according to NP_005182.1). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.3-1 μg/ml.
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti CD80 antibody, anti CD80 molecule antibody, a
• Research Area: Epigenetics, Infectious Diseases, Stem Cells
• Note: For research use only
• Entrez: 941
• Expiration Date: 12 months from date of receipt.

0.5 µg/ml staining of K562 (A) and U937 (B) cell lysate (35 µg protein in RIPA buffer). Detected by chemiluminescence.

3 ug/ml staining of Human Lymph Node (A), Bone Marrow (B) and 1.5 ug/ml Spleen (C) lysate (35 µg protein in RIPA buffer). Detected by chemiluminescence.

Flow cytometric analysis of paraformaldehyde fixed U937 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (1 ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

6 µg/ml staining of paraffin embedded Human Spleen. Heat induced antigen retrieval with citrate buffer pH6, HRP-staining.

Negative Control showing staining of paraffin embedded Human Spleen, with no primary antibody.

5 µg/ml staining of paraffin embedded Human Tonsil. Heat induced antigen retrieval with citrate buffer pH6, HRP-staining.

Negative Control showing staining of paraffin embedded Human Tonsil, with no primary antibody.