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Goat anti-CD80 Antibody

Catalog Number: orb20356

DispatchUsually dispatched within 5-10 working days
$ 500.00
Catalog Numberorb20356
CategoryAntibodies
DescriptionGoat polyclonal to CD80
Species/HostGoat
ClonalityPolyclonal
Tested applicationsELISA, FC, IHC, WB
ReactivityHuman
Dilution rangeELISA: 1:4000, WB: 0.3-1 μg/ml
ConjugationUnconjugated
MW33
TargetCD80
Entrez941
Protein SequenceHLRVNQTFNWNTTK
RRIDAB_10748963
StorageAliquot and store at -20°C. Minimize freezing and thawing.
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Alternative namesanti CD80 antibody, anti CD80 molecule antibody, a
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NoteFor research use only
Application notesELISA: Peptide ELISA: antibody detection limit dilution 1:4000.WB: Approx 60kDa band observed in Human Lymph Node and Human Tonsil lysates (calculated MW of 33.0kDa according to NP_005182.1). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.3-1 μg/ml.
Expiration Date12 months from date of receipt.
Goat anti-CD80 Antibody

0.5 µg/ml staining of K562 (A) and U937 (B) cell lysate (35 µg protein in RIPA buffer). Detected by chemiluminescence.

Goat anti-CD80 Antibody

3 ug/ml staining of Human Lymph Node (A), Bone Marrow (B) and 1.5 ug/ml Spleen (C) lysate (35 µg protein in RIPA buffer). Detected by chemiluminescence.

Goat anti-CD80 Antibody

Flow cytometric analysis of paraformaldehyde fixed U937 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (1 ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Goat anti-CD80 Antibody

6 µg/ml staining of paraffin embedded Human Spleen. Heat induced antigen retrieval with citrate buffer pH6, HRP-staining.

Goat anti-CD80 Antibody

Negative Control showing staining of paraffin embedded Human Spleen, with no primary antibody.

Goat anti-CD80 Antibody

5 µg/ml staining of paraffin embedded Human Tonsil. Heat induced antigen retrieval with citrate buffer pH6, HRP-staining.

Goat anti-CD80 Antibody

Negative Control showing staining of paraffin embedded Human Tonsil, with no primary antibody.