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Item 1 of 7
Item 1 of 7
Goat anti-CD80 Antibody
Catalog Number: orb20356
| Catalog Number | orb20356 |
|---|---|
| Category | Antibodies |
| Description | Goat polyclonal to CD80 |
| Target | CD80 |
| Clonality | Polyclonal |
| Species/Host | Goat |
| Conjugation | Unconjugated |
| Reactivity | Human |
| Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
| Protein Sequence | HLRVNQTFNWNTTK |
| RRID | AB_10748963 |
| MW | 33 |
| Tested applications | ELISA, FC, IHC, WB |
| Dilution range | ELISA: 1:4000, WB: 0.3-1 μg/ml |
| Application notes | ELISA: Peptide ELISA: antibody detection limit dilution 1:4000.WB: Approx 60kDa band observed in Human Lymph Node and Human Tonsil lysates (calculated MW of 33.0kDa according to NP_005182.1). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.3-1 μg/ml. |
| Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
| Alternative names | anti CD80 antibody, anti CD80 molecule antibody, a Read more... |
| Note | For research use only |
| Entrez | 941 |
0.5 µg/ml staining of K562 (A) and U937 (B) cell lysate (35 µg protein in RIPA buffer). Detected by chemiluminescence.
3 ug/ml staining of Human Lymph Node (A), Bone Marrow (B) and 1.5 ug/ml Spleen (C) lysate (35 µg protein in RIPA buffer). Detected by chemiluminescence.
Flow cytometric analysis of paraformaldehyde fixed U937 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (1 ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
6 µg/ml staining of paraffin embedded Human Spleen. Heat induced antigen retrieval with citrate buffer pH6, HRP-staining.
Negative Control showing staining of paraffin embedded Human Spleen, with no primary antibody.
5 µg/ml staining of paraffin embedded Human Tonsil. Heat induced antigen retrieval with citrate buffer pH6, HRP-staining.
Negative Control showing staining of paraffin embedded Human Tonsil, with no primary antibody.
