CD28 antibody

• Catalog Number: orb378206
• Category: Antibodies
• Description: CD28 antibody
• Target: CD28
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Form/Appearance: 10 mM PBS, 0.02% sodium azide
• Concentration: - 100 μg (in 200 μl): 0.5 mg/ml- 200 μg (in 400 μl): 0.5 mg/ml
• Purity: Polyclonal antibodies are purified by peptide affinity chromatography
• Immunogen: KLH conjugated synthetic peptide derived from human CD28. Please contact us for the exact immunogen sequence. The peptide is available as orb12968.
• MW: 25 kDa
• Tested applications: ICC, IF, IHC-P, WB
• Dilution range: IF/ICC: 1:100-600, IHC-P: 1:100-600, WB: 1:200-1500
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Mahshad Entezam 1, Nader Bagheri 2, Amin Soltani 3, Sayedeh Azimeh Hosseini 4, Pegah Khosravian 5, Gordon A Ferns 6, Mahdi GhatrehSamani Enhanced antitumor immunity in breast cancer: Synergistic effects of ADAM10/ADAM17 inhibition, metabolic modulation, and camptothecin-loaded selenium nanoparticles Int J Pharm, (2024)

PubMed: 39675534

Mohammad Jahan-Mahin 1, Roya Askari 1, Amir Hossein Haghighi 1, Omid Khaiyat The effect of three types of water-based training protocols on thymus atrophy and specific indicators of cellular immune senescence in aged male rats Biogerontology ., (2025)

PubMed: 39832052

Applications

IHC

Reactivity

Rat

IHC-P staining of rat thyroid tissue using CD28 antibody (dilution at 2.5 ug/ml)

Immunohistochemical staining of paraffin embedded rat muscle tissue using CD28 antibody (primary antibody at 2.5 ug/ml)

Immunohistochemical staining of paraffin embedded human thyroid tumour tissue using CD28 antibody (primary antibody at 2.5 ug/ml)

Immunohistochemical staining of paraffin embedded rat muscle tissue using CD28 antibody (primary antibody at 2.5 ug/ml)

Wesstern blot analysis of rat lymph node (lane 1), rat spleen (lane 2), mouse thymus (lane 3), rat thyroid (lane 4), rat small intestines (lane 5), human thyroid cancer (lane 6) using CD28 antibody (1 ug/ml)

IHC-P staining of mouse spleen tissue using CD28 antibody (dilution at 2.5 ug/ml)

IHC-P image of rat thyroid tissue using anti-CD28 (dilution of primary antibody at 2.5 ug/ml)

IHC-P staining of human thyroid tumour tissue using CD28 antibody (dilution at 2.5 ug/ml)

WB analysis of rat lymph node (lane 1), rat spleen (lane 2), mouse thymus (lane 3), rat thyroid (lane 4), rat small intestines (lane 5), human thyroid cancer (lane 6) using CD28 antibody (1 ug/ml)

Immunofluorescence image of rat cartilage tissue using CD28 antibody (dilution at 2.5 ug/ml)

IF analysis of rat cartilage tissue using anti-CD28 (dilution of primary antibody at 2.5 ug/ml)