CD28 Antibody

SKU: orb749318

Description

This antibody recognizes a glycoprotein of 44-88kDa, which is identified as CD28. It is the critical Tcell co-stimulatory receptor which provides to the cell the important second activation signal by binding CD80 and CD86 that are expressed by antigen presenting cells. Besides its co-stimulation role, CD28 functions in preventing Tcells from anergic hyporesponsive state or from undergoing premature apoptotic cell death. it is also expressed on human fetal NK cells and some NK cell lines, whereas on murine NK cells the CD28 expression is much broader.

Research Area

Immunology & Inflammation

Images & Validation

• Tested Applications: FACS, Functional Studies, IF
• Dilution Range: Flow cytometry: 1-2ug/10^6 cells,Immunofluorescence: 1-2ug/ml,Functional studies (order BSA/sodium azide-free format)
• Reactivity: Human
• Application Notes:
  The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the CD28 antibody to be titered up or down for optimal performance.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG1, kappa
• Clone No.: CB28
• Immunogen: Recombinant human protein was used as the immunogen for this CD28 antibody.
• Purification: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only