Caspase-3 Rabbit mAb [KD Validated] Antibody

SKU: orb1951945

Description

Caspase-3 (CPP-32, Apoptain, Yama, SCA-1) is a critical executioner of apoptosis, as it is either partially or totally responsible for the proteolytic cleavage of many key proteins such as the nuclear enzyme poly(ADP-ribose) polymerase (PARP). Activation of caspase-3 requires proteolytic processing of its inactive zymogen into activated p17 and p12 fragments. Cleavage of caspase-3 requires aspartic acid at the P1 position.

Images & Validation

• Tested Applications: FC, ICC, IHC, IP, WB
• Dilution Range: WB 1:500~1:2000 IHC 1:50~1:200 ICC/IF 1:50~1:200 IP 1:50
• Reactivity: Human

Key Properties

• Antibody Type: Recombinant Antibody
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: Rabbit IgG
• Immunogen: A synthesized peptide derived from human Caspase-3
• Purification: Affinity-chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CASP3; Caspase-3; APOPAIN; CPP32; CPP32B; SCA-1; Caspase3;

All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.

Immunofluorescent analysis of Hela cells, using Caspase-3 Antibody.

Immunohistochemical analysis of paraffin-embedded human tonsil, using Caspase-3 Antibody.

Western blot analysis of Caspase-3 in HEK293 cell lysate.

Quick Database Links

UniProt

P42574