active + pro Caspase 3 Rabbit mAb [KD Validated]

• Catalog Number: orb1951680
• Category: Antibodies
• Description: This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce 2 subunits, large and small, that dimerize to form the active enzyme.
• Clonality: Recombinant
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Form/Appearance: Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Purification: Affinity-chromatography
• Immunogen: A synthesized peptide derived from human active + pro Caspase 3
• UniProt ID: P42574
• Tested applications: IHC, IP, WB
• Dilution range: WB 1:500~1:2000 IHC 1:50~1:200 IP 1:50
• Antibody Type: Recombinant Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Apopain precursor; CASP-3; CPP-32; CPP32; Caspase-
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.

Immunohistochemical analysis of paraffin-embedded human stomach, using active + pro Caspase 3 Antibody.

Western blot analysis of Calreticulin expression in (1) Jurkat cell lysate; (2) COLO cells lysate.

Western blot analysis of Calreticulin expression in (1) Jurkat cell lysate; (2) Jurkat cells treated with 1uM staurosporine lysate.