Caspase-9 Rabbit mAb [KD Validated]

• Catalog Number: orb1951460
• Category: Antibodies
• Description: Caspase-9 is an important member of the cysteine aspartic acid protease (caspase) family. Involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates caspase-3. Promotes DNA damage-induced apoptosis in a ABL1/c-Abl-dependent manner. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP).
• Species/Host: Rabbit
• Clonality: Recombinant
• Tested applications: ICC, IHC, IP, WB
• Reactivity: Human, Mouse
• Isotype: Rabbit IgG
• Immunogen: A synthesized peptide derived from human Caspase-9
• Antibody Type: Recombinant Antibody
• Dilution range: WB 1:500~1:2000 IHC 1:50~1:200 ICC/IF 1:50~1:200 IP 1:50
• Form/Appearance: Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Conjugation: Unconjugated
• UniProt ID: P55211
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: CASP 9; Caspase-9; CASP-9; Apoptotic protease Mch-
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.

Immunofluorescent analysis of HeLa cells treated with staurosporine, using Caspase-9 Antibody.

Immunohistochemical analysis of paraffin-embedded human liver, using Caspase-9 Antibody.

Western blot analysis of Caspase-9 expression in HeLa cell lysate.