Caspase-3 p12 Rabbit mAb [KD Validated] Antibody

SKU: orb1951459

Description

Caspase-3 is a critical executioner of apoptosis, as it is either partially or totally responsible for the proteolytic cleavage of many key proteins, such as the nuclear enzyme poly (ADP-ribose) polymerase (PARP). Involved in the activation cascade of caspases responsible for apoptosis execution.

Images & Validation

• Tested Applications: ICC, IHC, IP, WB
• Dilution Range: WB 1:500~1:1000 IHC 1:100~1:500 ICC/IF 1:50~1:200
• Reactivity: Human, Mouse, Rat

Key Properties

• Antibody Type: Recombinant Antibody
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: Rabbit IgG
• Immunogen: A synthesized peptide derived from human Caspase-3 p12
• Purification: Affinity-chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Apopain; CASP-3; CASP3; Caspase-3 subunit p12; CPP-32; Cysteine protease CPP32; Protein Yama; SCA-1; SREBP cleavage activity 1;

All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.

Immunofluorescent analysis of HeLa cells, using Caspase-3 p12 Antibody.

Immunohistochemical analysis of paraffin-embedded human kidney, using Caspase-3 p12 Antibody.

Western blot analysis of Caspase-3 p12 expression in HeLa cell treated with staurosporine lysate.

Quick Database Links

UniProt

P42574