CAMK2A Antibody

• Catalog Number: orb1249621
• Category: Antibodies
• Description: CAMK2A Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, FC, IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Immunogen: The immunogen for this antibody is: C-PRTAQSEETRVWHR
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 58-60kDa band observed in Human (Cerebellum), Mouse and Rat Brain lysates (calculated MW of 55.3kDa according to Human NP_057065.2, and 54.1kDa according to to Mouse NP_803126.1 and Rat NP_037052.1). An additional band of 50kDa was observed in some Rat Brain lysates and wss successfully blocked by incubation with the immunizing peptide. The observed molecular weights correspond to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.03--0.3ug/ml. Primary incubation 1 hour at room temperature.Immunohistochemistry:In paraffin embedded Human Brain Cortex shows granular staining in the periphery of pyramydal cell bodies. Recommended concentration: 3-6ug/ml. Paraffin embedded Human Adrenal Gland. Recommended concentration: 3.75ug/ml.Immunofluorescence: Strong expression of the protein seen in the membranes of U2OS and U251 cells. Recommended concentration: 10ug/ml. Flow Cytometry: Flow cytometric analysis of Kelly cells. Recommended concentration: 10ug/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: CAMK2A
• UniProt ID: Q9UQM7
• NCBI: NP_741960.1, NP_057065.2
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: CAMK2A, calcium/calmodulin-dependent protein kinas
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 58-60kDa band observed in Human (Cerebellum), Mouse and Rat Brain lysates (calculated MW of 55.3kDa according to Human NP_057065.2, and 54.1kDa according to to Mouse NP_803126.1 and Rat NP_037052.1). An additional band of 50kDa was observed in some Rat Brain lysates and wss successfully blocked by incubation with the immunizing peptide. The observed molecular weights correspond to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.03--0.3ug/ml. Primary incubation 1 hour at room temperature.Immunohistochemistry:In paraffin embedded Human Brain Cortex shows granular staining in the periphery of pyramydal cell bodies. Recommended concentration: 3-6ug/ml. Paraffin embedded Human Adrenal Gland. Recommended concentration: 3.75ug/ml.Immunofluorescence: Strong expression of the protein seen in the membranes of U2OS and U251 cells. Recommended concentration: 10ug/ml. Flow Cytometry: Flow cytometric analysis of Kelly cells. Recommended concentration: 10ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1249621 (3.8 ug/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

orb1249621 (3.75 ug/ml) staining of paraffin embedded Human Adrenal Gland. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

orb1249621 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue).

orb1249621 Immunofluorescence analysis of paraformaldehyde fixed U251 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue).

orb1249621 Flow cytometric analysis of paraformaldehyde fixed Kelly cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (1 ug/ml). IgG control: Unimmunized goat IgG (black line).