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Phospho-CaMK2 alpha (Tyr230) Rabbit Polyclonal Antibody

Catalog Number: orb4756

DispatchUsually dispatched within 5-10 working days
$ 210.00
Catalog Numberorb4756
CategoryAntibodies
DescriptionPhospho-CaMK2 alpha (Tyr230) Rabbit Polyclonal Antibody
TargetCAMK2A
ClonalityPolyclonal
Species/HostRabbit
IsotypeIgG
ConjugationUnconjugated
ReactivityHuman, Mouse, Rat
Predicted ReactivityBovine, Canine, Equine, Gallus, Porcine, Rabbit, Sheep
Form/AppearanceLiquid
Concentration1mg/ml
Buffer/Preservatives0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
ImmunogenKLH conjugated Synthesised phosphopeptide derived from human CaMK2 alpha around the phosphorylation site of Tyr231 GA(p-Y)DF
UniProt IDQ9UQM7
RRIDAB_10937855
MW54 kDa
Tested applicationsFC, IF, IHC-Fr, IHC-P, WB
Dilution rangeWB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=2ug/Test
Antibody TypePrimary Antibody
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Alternative namesphospho-CaMK2B(Tyr230); CaMK2B(phospho Tyr230); al
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NoteFor research use only
Phospho-CaMK2 alpha (Tyr230) Rabbit Polyclonal Antibody

Blank control: K562. Primary Antibody (green line): Rabbit Anti-phospho-CaMK2 alpha (Tyr230) antibody (orb4756), dilution: 2 ug/Test, Secondary Antibody: Goat anti-rabbit IgG-FITC, dilution: 0.5 ug/Test. Protocol The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Phospho-CaMK2 alpha (Tyr230) Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (Human gastric), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (p-CaMK2 alpha (Tyr230)) Polyclonal Antibody, Unconjugated (orb4756) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Phospho-CaMK2 alpha (Tyr230) Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (Human kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (p-CaMK2 alpha (Tyr230)) Polyclonal Antibody, Unconjugated (orb4756) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Phospho-CaMK2 alpha (Tyr230) Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (Mouse stomach), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (p-CaMK2 alpha) Polyclonal Antibody, Unconjugated (orb4756) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Phospho-CaMK2 alpha (Tyr230) Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (rat cerebellum), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (p-CaMK2 alpha (Tyr230)) Polyclonal Antibody, Unconjugated (orb4756) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Phospho-CaMK2 alpha (Tyr230) Rabbit Polyclonal Antibody

Sample: Cerebrum (Rat) cell Lysate at 40 ug, Primary: Anti-phospho-CaMK2 alpha (Tyr230) (orb4756) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 54 kD, Observed band size: 54 kD.

Phospho-CaMK2 alpha (Tyr230) Rabbit Polyclonal Antibody

Sample: Lane 1: Mouse Muscle tissue lysates, Lane 2: Mouse Heart tissue lysates, Primary: Anti-phospho-CaMK2 alpha (Tyr230) (orb4756) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 54 kDa, Observed band size: 50 kDa.