CaM Kinase II (Thr286) Antibody

SKU: orb319474

Description

Our Anti-CaM Kinase II (Thr286) rabbit polyclonal phosphospecific primary antibody from Biorbyt is produced in-house. It detects human, mouse, rabbit, and rat CaM Kinase II (Thr286) and is antigen affinity purified from pooled serum.

Research Area

Cell Biology, Neuroscience, Pharmacology & Drug Discovery, Signal Transduction

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution Range: WB: 0.736111111, IHC: 0.736111111
• Reactivity: Human, Mouse, Rabbit, Rat
• Predicted Reactivity: Zebrafish

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr286 of rat CaMKII, conjugated to keyhole limpet hemocyanin (KLH).
• Target: CaM Kinase II (Thr286)
• Molecular Weight: 50/60
• Purification: Antigen Affinity Purified from Pooled Serum
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml rAlbumin and 50% glycerol.
• Concentration: Lot Dependent
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Calcium/calmodulin dependent protein kinase II alpha antibody, Calcium/calmodulin dependent protein kinase II beta antibody, Calcium/calmodulin dependent protein kinase II delta antibody, Calcium/calmodulin dependent protein kinase II gamma antibody, Calcium/calmodulin-dependent protein kinase type II subunit alpha antibody, CaM kinase II alpha antibody, CaM kinase II antibody, CaM kinase II beta antibody, CaM kinase II delta antibody, CaM kinase II gamma antibody, CaM kinase II subunit alpha antibody, CaMK-II subunit alpha antibody, CAMK2 antibody, Camk2a antibody, CAMK2B antibody, CAMK2D antibody, CAMK2G antibody, CAMKA antibody, KCC2A_HUMAN antibody

Astrocytes (1 × 106 cells/ml) were cultured for 24 h without treatment or with METH (10 μM), clade B gp120 (50 ng/ ml), or clade C gp120 (50 ng/ ml) alone or METH in combination with clade B or C gp120. After incubation, the cells were fixed, permeabilized, and stained for (A) DRD-2, (B) phospho-CaMK II (Cat. orb319474) (C) phospho-CaMK IV (green) or GFPA (red) and DAPI (blue). The cells were imaged using a confocal microscope at 630x and analyzed using immunocytochemistry.

Depicts increased CaMKII autophosphorylation at Threonine 287 (Cat. orb319474, 1:1000) (normalized to CaMKIIδ expression) following ATX-II exposure in WT and KI cells.

Representative immunoblot and quantification of in vitro kinase reactions at 30 °C in 1 mM ATP measuring CaMKIIα WT and F89G phosphorylation of T286 (Cat. orb319474, 1:2500).

Representative immunoblot and quantification of in vitro kinase reactions at 30 °C in 1 mM ATP measuring AS283-mediated inhibition of CaMKII phosphorylation of GluA1 S831 (pS831) (Cat. orb319552, 1:2000) and autophosphorylation of CaMKII T286 (pT286) (Cat. orb319474, 1:2500) with varying concentrations of AS283 (0.41–10 μM). For a negative control (Neg.), ATP was omitted.

Western blot of rat brain lysate showing specific immunolabeling of the ~50 kDa α- and the ~60 kDa β-CaM Kinase II phosphorylated at Thr286 in the first lane (-). Phosphospecificity is shown in the second lane (+) where the immunolabeling is completely eliminated by blot treatment with lambda phosphatase (λ-Ptase, 1200 units for 30 minutes).

Western immunoblot measuring levels of T286 phosphorylation of CaMKII (Cat. orb319474,1:1000) normalized to total CaMKII in the membrane fraction of whole hippocampi from sham and CA/CPR operated mice.

Quick Database Links

Gene Symbol

CaM Kinase II (Thr286)

UniProt

P11275