CAMK2A + CAMK2B + CAMK2D Rabbit Polyclonal Antibody

• Catalog Number: orb10224
• Category: Antibodies
• Description: CAMK2A + CAMK2B + CAMK2D Rabbit Polyclonal Antibody
• Target: CAMK2A
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Mouse, Rat
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Purification: Affinity purified by Protein A
• Immunogen: KLH conjugated synthetic peptide derived from human CaMK2 beta/ gamma/ delta (1-100/479aa)
• UniProt ID: Q9UQM7
• RRID: AB_10762843
• MW: 53-73 kDa
• Tested applications: ICC, IF, IHC-Fr, IHC-P
• Dilution range: IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:50, IF=1:100-500
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: CaMK2 beta+gamma+delta; CaMK2 beta; CaMK2 gamma; C
• Research Area: Cardiovascular Research, Hypoxia, Signaling Transd
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

4% Paraformaldehyde-fixed SH-SY5Y (H) cell, Triton X-100 at r.t. for 20 min, Antibody incubation with (CAMK2A + CAMK2B + CAMK2D) polyclonal Antibody, unconjugated (orb10224) 1:50, 90 min at 37°C, followed by conjugated Goat Anti-Rabbit IgG antibody (green) at 37°C for 90 min, DAPI (blue) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.

Paraformaldehyde-fixed, paraffin embedded (human brain glioma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (CAMK2A + CAMK2B + CAMK2D) Polyclonal Antibody, Unconjugated (orb10224) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (CAMK2A + CAMK2B + CAMK2D) Polyclonal Antibody, Unconjugated (orb10224) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (CaMK2b) Polyclonal Antibody, Unconjugated (orb10224) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Rat cerebellum), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (CaMK2b) Polyclonal Antibody, Unconjugated (orb10224) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Lung (Mouse) Lysate at 40 ug, Primary: Anti-CAMK2A+CAMK2B+CAMK2D (orb10224) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/10000 dilution, Predicted band size: 53-73 kD, Observed band size: 60 kD.

Sample: Small intestine (Mouse) Lysate at 40 ug, Primary: Anti-CAMK2A+CAMK2B+CAMK2D (orb10224) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/10000 dilution, Predicted band size: 53-73 kD, Observed band size: 60 kD.