Beta Tubulin Antibody Loading Control

• Catalog Number: orb248111
• Category: Antibodies
• Description: There are five mammalian members of the tubulin family: alpha, beta, gamma, delta, and epsilon. Heterodimers of alpha and beta tubulin make up microtubules, which along with microfilaments and intermediate filaments, make up the cytoskeleton. Due to its ubiquitous presence in mammalian cells, it is often used a loading control protein in western blot. An antibody to beta-tubulin is used to demonstrate equivalent protein loading in different gel lanes, typically transfected vs mock or non transfected cell lysate.
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: WB
• Reactivity: Canine, Human, Mouse, Primate, Rat
• Isotype: Rabbit IgG
• Immunogen: A recombinant protein fragment from the C-terminal region of human beta tubulin was used as the immunogen for this antibody.
• Dilution range: Western blot: 2-4ug/ml
• Purity: Protein A Affinity Chromatography
• Conjugation: Unconjugated
• Formula: 0.5 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This beta Tubulin antibody loading control is available for research use only.
• Entrez: 203068
• Storage: Aliquot the loading control antibody and store frozen at -20°C or colder to avoid repeated freeze-thaw cycles.
• Buffer/Preservatives: 1X PBS, pH 7.4
• Note: For research use only
• Application notes: Provided assay concentrations are suggestions only, antibody titration may be required for optimal results.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of human samples using beta Tubulinantibody (primary antibody dilution at: at 2 ug/ml)

Western blot analysis of mouse samples using beta Tubulinantibody (primary antibody dilution at: at 2 ug/ml)