Loading Control Antibody Panel

SKU: orb3130873

Description

The Loading Control Antibody Panel contains a curated set of antibodies targeting constitutively expressed housekeeping proteins. This panel enables accurate normalization of protein expression data across a diverse range of cell lines and tissues, including targets with molecular weights ranging from ~15 to 100 kDa such as GAPDH, beta-Actin, and Tubulin.

Images & Validation

• Tested Applications: IF, IHC, WB
• Dilution Range: Western Blot 1:3000 - 1:10000, Immunochemistry 1:100 - 1:500
• Reactivity: Human, Mouse, Rat

Key Properties

• Isotype: Rabbit-IgG / Mouse-IgG / Goat-IgG
• Immunogen: Synthetic peptide
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

GAPDH antibody Western blot analysis of cell extracts using GAPDH orb1294414 at 1/5000 dilution.

Beta-tubulin antibody Immunochemistry analysis of cell extracts using Beta-tubulin orb1294417 at 1/10000 dilution.

Beta-actin antibody Immunochemistry analysis of cell extracts using Beta-actin orb1294415 at 1/3000 dilution.

Alpha-tubulin monoclonal Immunochemistry analysis of cell extracts using Alpha-tubulin orb1294282 at 1/3000 dilution.

Goat anti-rabbit IgG (H+L), HRP Western blot analysis of cell and tissue extracts using Anti-rabbit IgG (H+L), HRP at 1/10000 dilution.