Beta tubulin Rabbit Polyclonal Antibody (Loading Control)

SKU: orb100281

Description

Beta tubulin Rabbit Polyclonal Antibody (Loading Control)

Images & Validation

• Tested Applications: FC, IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:10000-100000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1ug/Test
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Rabbit

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human tubulin Beta (61-160/444aa)
• Target: TUBB
• Molecular Weight: 50 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

beta 4 tubulin; Tubulin-beta; Tubulin beta; beta 5 tubulin; betaTubulin; beta-Tubulin; dJ40E16.7; TUBB; TUBB2; TUBB2A; TUBB5; tubulin beta 2A; Tubulin beta chain; Tubulin beta-5 chain; TUBB4A; TUBB4; Tubulin 5 beta; Tubulin beta-4 chain; TBB4A_HUMAN; Tubulin beta-4A chain.

Blank control: HL-60. Primary Antibody (green line): Rabbit Anti-Beta tubulin antibody (orb100281), Dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-AF488, Dilution: 1 µg/test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Sample: Lane 1, 3, 5: Mouse Cerebellum tissue lysates, Lane 2, 4, 6: Rat Cerebellum tissue lysates, Primary: Anti-Beta tubulin (orb100281) at 1/10000~1/50000 dilution, Secondary: HRP conjugated Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 50 kDa, Observed band size: 48 kDa.

Quick Database Links

Gene Symbol

TUBB

UniProt

P07437

References

O Özcan, Y Yazır, G Duruksu, A Öztürk, KC Kılıç Investigation of Oxidative Stress and Mitochondrial Damage on Rat Derived Neural Stem Cells in 3D Organoid Models researchsquare.com, (2024)

PubMed:

Poonam Dhiman 1, Rajneesh Kumar 1, Damanpreet Singh Neuronal nitric oxide synthase activation by tadalafil protects neurological impairments in a zebrafish larva model of hyperammonemia Life Sci ., (2024)

PubMed: 39710060

Applications

WB

Rochana Pramanik 1, Sreya Chattopadhyay 1, Biswadev Bishayi Dual neutralization of TGF-β and IL-21 regulates Th17/Treg balance by suppressing inflammatory signalling in the splenic lymphocytes of Staphylococcus aureus infection-induced septic arthritic mice Immunol Res ., (2025)

PubMed: 39831928

Gopinath Mukherjee 1, Sharmistha Samanta 1, Biswadev Bishayi 2 TLR-4Ab and IFNγAb with exogenous IL-10 treated LPS induced mice shown differential inflammatory response upon RANKL-M-CSF stimulation in Resident Bone Marrow Cells Microb Pathog, (2025)

PubMed: 40023455

Applications

WB

Sharmistha Samanta , Gopinath Mukherjee , Sreya Chattopadhyay , Biswadev Bishay Concomitant inhibition of TLR4 and NLRP3 inflammasome in macrophages regulates lipopolysaccharide induced inflammation The Microbe, (2025)

PubMed:

Applications

WB

Reactivity

Mouse

Rajneesh Kumar 1 2, Poonam Dhiman 1 2, Shiv Kumar Saini 1 2, Damanpreet Singh Downregulation of Glycogen Synthase Kinase-3 Beta by Urtica dioica L. Contributes to Neuroprotection Against Aluminum Chloride-Mediated Neurotoxicity in Zebrafish Larvae Mol Neurobiol ., (2025)

PubMed: 40770461