Beta tubulin Mouse Monoclonal Antibody (Loading Control)

• Catalog Number: orb499644
• Category: Antibodies
• Description: Beta tubulin Mouse Monoclonal Antibody (Loading Control)
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 7G3
• Tested applications: ICC, IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Bovine, Canine, Gallus, Guinea pig, Insect, Monkey, Porcine, Rabbit, Sheep, Yeast
• Reactivity: Human, Mouse, Rat
• Isotype: IgG1
• Immunogen: KLH conjugated synthetic peptide derived from human Beta tubulin
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:5000-20000, IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:100, IF=1:100-500
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 50 kDa
• Target: TUBB
• UniProt ID: P07437
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: beta 4 tubulin; beta 5 tubulin; beta Ib tubulin; b
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Hela cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (Beta tubulin) monoclonal Antibody, Unconjugated (orb499644) 1:100, 90 minutes at 37°C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

Paraformaldehyde-fixed, paraffin embedded (mouse cerebellum), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Beta tubulin (Loading Control)) Monoclonal Antibody, Unconjugated (ascites of orb499644 7G3) at 1:2000 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructions and DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Beta tubulin (Loading Control)) Monoclonal Antibody, Unconjugated (orb499644) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

Sample: Cerebrum (Mouse) Lysate at 40 ug, Primary: Anti-Beta tubulin (orb499644) at 1/1000~20000 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 55 kD, Observed band size: 55 kD.

Sample: Hela (Human) Cell Lysate at 30 ug, NIH/3T3 (Mouse) Cell Lysate at 30 ug, HL60 (Human) Cell Lysate at 30 ug, SH-SY5Y (Human) Cell Lysate at 30 ug, Cerebellum (Mouse) Lysate at 40 ug, Cerebellum (Rat) Lysate at 40 ug, Cerebrum (Mouse) Lysate at 40 ug, Cerebrum (Rat) Lysate at 40 ug, Primary: Anti-Beta tubulin (orb499644) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 50 kD, Observed band size: 50 kD.

Sample: Hela (Human) Lysate at 40 ug, Primary: Anti-Beta tubulin (orb499644) at 1/2000~1/20000 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 55 kD, Observed band size: 55 kD.