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Phospho-Beta-Catenin (Ser33 + Ser37) Rabbit Polyclonal Antibody

Catalog Number: orb5768

DispatchUsually dispatched within 5-10 working days
$ 210.00
Catalog Numberorb5768
CategoryAntibodies
DescriptionPhospho-Beta-Catenin (Ser33 + Ser37) Rabbit Polyclonal Antibody
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsFC, IF, IHC-Fr, IHC-P
Predicted ReactivityGallus, Porcine, Rabbit
ReactivityHuman, Mouse, Rat
IsotypeIgG
ImmunogenKLH conjugated Synthesised phosphopeptide derived from human Beta-Catenin around the phosphorylation site of Ser33/37 LD(p-S)GIH(p-S)GA
Antibody TypePrimary Antibody
Concentration1mg/ml
Dilution rangeIHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=0.2μg /test
Form/AppearanceLiquid
ConjugationUnconjugated
MW86 kDa
TargetCTNNB1
UniProt IDP35222
RRIDAB_10932097
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Alternative namesbeta Catenin(phospho S33/37); beta Catenin(phospho
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NoteFor research use only
Expiration Date12 months from date of receipt.
Phospho-Beta-Catenin (Ser33 + Ser37) Rabbit Polyclonal Antibody

Blank control (blue line): Hela (fixed with 70% ethanol (Overnight at 4°C) and then permeabilized with 90% ice-cold methanol for 30 min on ice). Primary Antibody (green line): Rabbit Anti-Phospho-Beta-Catenin (Ser33+37) antibody (orb5768), dilution: 0.2 µg/10^6 cells. Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC, dilution: 1 µg/Test.

Phospho-Beta-Catenin (Ser33 + Ser37) Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Beta-Catenin (Ser33 + Ser37)) Polyclonal Antibody, Unconjugated (orb5768) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Phospho-Beta-Catenin (Ser33 + Ser37) Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Phospho-Beta-Catenin (Ser33 + Ser37)) Polyclonal Antibody, Unconjugated (orb5768) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Phospho-Beta-Catenin (Ser33 + Ser37) Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (mouse intestine tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Beta-Catenin (Ser33 + Ser37)) Polyclonal Antibody, Unconjugated (orb5768) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Phospho-Beta-Catenin (Ser33 + Ser37) Rabbit Polyclonal Antibody

Tissue/Cell: rat brain tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-Phospho-Beta-Catenin (Ser33+37) Polyclonal Antibody, Unconjugated (orb5768) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Phospho-Beta-Catenin (Ser33 + Ser37) Rabbit Polyclonal Antibody

Tissue/Cell: rat brain tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-Phospho-Beta-Catenin (Ser33+37) Polyclonal Antibody, Unconjugated (orb5768) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

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Phospho-Beta-Catenin (Ser33 + Ser37) Rabbit Polyclonal Antibody (orb5768)

Worked really well for us!

- Neha Jain/University of Illinois at Chicago

Applications

ELISA

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Phospho-Beta-Catenin (Ser33 + Ser37) Rabbit Polyclonal Antibody (orb5768)

The antibody worked well.

- Kiran K. Velpula/University of Illinois at Chicago

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WB

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  • 5 stars

Phospho-Beta-Catenin (Ser33 + Ser37) Rabbit Polyclonal Antibody (orb5768)

The antibody worked well for WB.

- Collin Labak

Applications

WB

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  • 4 stars