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Catalog Number | orb5768 |
---|---|
Category | Antibodies |
Description | Phospho-Beta-Catenin (Ser33 + Ser37) Rabbit Polyclonal Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | FC, IF, IHC-Fr, IHC-P |
Predicted Reactivity | Gallus, Porcine, Rabbit |
Reactivity | Human, Mouse, Rat |
Isotype | IgG |
Immunogen | KLH conjugated Synthesised phosphopeptide derived from human Beta-Catenin around the phosphorylation site of Ser33/37 LD(p-S)GIH(p-S)GA |
Antibody Type | Primary Antibody |
Concentration | 1mg/ml |
Dilution range | IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=0.2μg /test |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | 86 kDa |
Target | CTNNB1 |
UniProt ID | P35222 |
RRID | AB_10932097 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
Alternative names | beta Catenin(phospho S33/37); beta Catenin(phospho Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Blank control (blue line): Hela (fixed with 70% ethanol (Overnight at 4°C) and then permeabilized with 90% ice-cold methanol for 30 min on ice). Primary Antibody (green line): Rabbit Anti-Phospho-Beta-Catenin (Ser33+37) antibody (orb5768), dilution: 0.2 µg/10^6 cells. Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC, dilution: 1 µg/Test.
Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Beta-Catenin (Ser33 + Ser37)) Polyclonal Antibody, Unconjugated (orb5768) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Phospho-Beta-Catenin (Ser33 + Ser37)) Polyclonal Antibody, Unconjugated (orb5768) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse intestine tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Beta-Catenin (Ser33 + Ser37)) Polyclonal Antibody, Unconjugated (orb5768) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Tissue/Cell: rat brain tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-Phospho-Beta-Catenin (Ser33+37) Polyclonal Antibody, Unconjugated (orb5768) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
Tissue/Cell: rat brain tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-Phospho-Beta-Catenin (Ser33+37) Polyclonal Antibody, Unconjugated (orb5768) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
FC, IF | |
Gallus, Porcine, Rabbit | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Cy5 |
IHC-Fr, IHC-P | |
Gallus, Porcine, Rabbit | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
AP |
FC, IF | |
Gallus, Porcine, Rabbit | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
RBITC |
FC, IF | |
Gallus, Porcine, Rabbit | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Cy5.5 |
FC, IF | |
Gallus, Porcine, Rabbit | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Cy7 |
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Worked really well for us!
- Neha Jain/University of Illinois at Chicago
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The antibody worked well.
- Kiran K. Velpula/University of Illinois at Chicago
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The antibody worked well for WB.
- Collin Labak
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