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Description
Research Area
Musculoskeletal & Connective Tissue Research
Images & Validation
−Item 1 of 2
| Tested Applications | ELISA, IF, IHC, WB |
|---|---|
| Dilution Range | WB: 1:1000 - 1:10000; IHC: 1:100 - 1:1000; IF: 1:50 - 1:200 |
| Reactivity | Human, Mouse, Rat |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG2a |
| Immunogen | actin, beta |
| Target | beta actin |
| Molecular Weight | 42 kDa |
| Purity | ≥95% as determined by SDS-PAGE |
| Purification | Protein A+G purified |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | liquid |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−ACTB, Actin, actin, beta, Actin, cytoplasmic 1, B actin, Beta actin, F actin, β actin
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Hela cells were subjected to SDS PAGE followed by western blot with orb625113 (ACTB antibody) at dilution of 1:5000.
Immunohistochemistry of paraffin-embedded rat heart slide using orb625113 (beta actin Antibody) at dilution of 1:200.
Quick Database Links
Gene Symbol
beta actin
UniProt
UniProt Details
− No UniProt data available
Documents Download
Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
IF
Immunofluorescence
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
beta actin Mouse Monoclonal Antibody (orb625113)
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