Beta-Actin Antibody

SKU: orb1939420

Description

Mouse Monoclonal Antibody (Mab)

Research Area

Cancer Biology; Musculoskeletal & Connective Tissue Research

Images & Validation

• Tested Applications: FC, IF, IHC-P, WB
• Dilution Range: WB - 1:1000, IF - 1:10-50, IHC-P - 1:25, FC - 1:25, IHC - 1:50
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Equine, Other, Porcine, Sheep

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1,Igk
• Clone No.: B359EV48X3X3
• Immunogen: This ACTB Monoclonal antibody is generated from mouse immunized with ACTB recombinant protein.
• Target: ACTB
• Molecular Weight: 41737 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Actin, cytoplasmic 1, Beta-actin, Actin, cytoplasmic 1, N-terminally processed, ACTB

All lanes: Anti-ACTB Antibody at 1:1000 dilution. Lane 1: A431 whole cell lysate. Lane 2: C2C12 whole cell lysate. Lane 3: C6 whole cell lysate. Lane 4: Hela whole cell lysate. Lane 5: MCF-7 whole cell lysate.Lysates/proteins at 20 μg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 42 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-ACTB Antibody at 1:1000 dilution. Lane 1: Hela whole cell lysate. Lane 2: HepG2 whole cell lysate. Lane 3: NIH-3T3 whole cell lysate.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 42 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-ACTB Antibody at 1:2000 dilution + C6 cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 41 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Confocal immunofluorescent analysis of ACTB Antibody with Hela cell followed by Alexa Fluor 488-conjugated goat anti-mouse lgG (green). DAPI was used to stain the cell nuclear (blue).

Immunohistochemical analysis of paraffin-embedded H.skeletal muscle section using Beta-Actin Antibody. diluted at 1:25 dilution. A peroxidase-conjugated goat anti-mouse IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.

Staining ACTB in human heart tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary Antibody.

Western blot analysis of anti-ACTB Antibody in K562, HL-60, Hela cell line, mouse spleen, mouse liver tissue lysates, mouse NIH-3T3 cell line lysate and mouse cerebellum, mouse brain tissue lysates (35 μg/lane). ACTB (arrow) was detected using the purified Mab.

Quick Database Links

Gene Symbol

ACTB

UniProt

P60709

RefSeq (Protein):NP_001092.1