Beta-Actin Mouse Monoclonal Antibody (Loading Control)

SKU: orb499643

Description

Beta-Actin Mouse Monoclonal Antibody (Loading Control)

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:1000-5000, IHC-P=1:200-2000, IHC-F=1:200-2000, IF=1:200-2000
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Canine, Gallus, Hamster, Monkey, Rabbit

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG
• Clone No.: 1A2
• Immunogen: Synthetic MAP peptide derived from human beta-Actin
• Target: ACTB
• Molecular Weight: 42 kDa
• Purification: Affinity purified by Protein G

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

ACTB_HUMAN; Actin, cytoplasmic 1; ACTB; EC:3.6.4.- ; Beta actin; Actin, cytoplasmic 1, N-terminally processed; actin beta; BNS; CSMH; DDS1; THC8; BKRNS; BRWS1; PS1TP5BP1; Actin cytoplasmic 1; β actin; β-actin; βactin;

25 ug total protein per lane of various lysates probed with beta-Actin monoclonal antibody, unconjugated (orb499643) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Blank control: Hela. Primary Antibody (green line): Mouse Anti-beta-Actin antibody (orb499643), dilution: 1 ug/Test, Secondary Antibody: Goat anti-Mouse IgG-FITC, dilution: 0.5 ug/Test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Paraformaldehyde-fixed, paraffin embedded (mouse colon), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (beta-Actin (Loading Control)) Monoclonal Antibody, Unconjugated (orb499643) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (mouse stomach tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (beta-Actin) Monoclonal Antibody, Unconjugated (ascites of orb499643) at 1:2000 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat colon), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (beta-Actin (Loading Control)) Monoclonal Antibody, Unconjugated (orb499643) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

Quick Database Links

Gene Symbol

ACTB

UniProt

P60709