Beta Actin Antibody Loading Control

• Catalog Number: orb55974
• Category: Antibodies
• Description: Actins proteins are highly conserved and involved in cellular structure, integrity and motility. There are three main isoforms of actin in vertebrates: alpha, beta and gamma. Alpha actins are found in muscle cells while beta and gamma actins are non-muscle actins. Beta actin is commonly used as a loading control protein in western blot. An antibody to beta actin is used to demonstrate equivalent protein loading in different gel lanes, typically transfected vs mock or non transfected cell lysate.
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: WB
• Reactivity: Canine, Gallus, Human, Monkey, Mouse, Rabbit, Rat
• Isotype: Rabbit IgG
• Immunogen: A recombinant protein fragment from the N-terminus of human beta Actin was used as the immunogen for this antibody.
• Dilution range: Western blot: 1-3ug/ml
• Purity: Protein A Affinity Chromatography
• Conjugation: Unconjugated
• Formula: 0.5 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This beta Actin antibody loading control is available for research use only.
• Entrez: 60
• Storage: Aliquot the loading control antibody and store frozen at -20°C or colder to avoid repeated freeze-thaw cycles.
• Buffer/Preservatives: 1X PBS, pH 7.4
• Note: For research use only
• Application notes: Provided assay concentrations are suggestions only, beta Actin antibody titration may be required for optimal results.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of Human samples using beta Actin antibody

Western blot analysis of Mouse samples using beta Actin antibody