Beta Actin Antibody Loading Control

SKU: orb55974

Description

Actins proteins are highly conserved and involved in cellular structure, integrity and motility. There are three main isoforms of actin in vertebrates: alpha, beta and gamma. Alpha actins are found in muscle cells while beta and gamma actins are non-muscle actins. Beta actin is commonly used as a loading control protein in western blot. An antibody to beta actin is used to demonstrate equivalent protein loading in different gel lanes, typically transfected vs mock or non transfected cell lysate.

Research Area

Musculoskeletal & Connective Tissue Research

Images & Validation

• Tested Applications: WB
• Dilution Range: Western blot: 1-3ug/ml
• Reactivity: Canine, Gallus, Human, Monkey, Mouse, Rabbit, Rat
• Application Notes:
  Provided assay concentrations are suggestions only, beta Actin antibody titration may be required for optimal results.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Immunogen: A recombinant protein fragment from the N-terminus of human beta Actin was used as the immunogen for this antibody.
• Purification: Protein A Affinity Chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.5 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin, 0.05% sodium azide
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Western blot testing of human samples with beta Actin antibody at 1 ug/ml.

Western blot testing of mouse samples with beta Actin antibody at 1 ug/ml.