ACTB Antibody

SKU: orb420310

Description

beta Actin antibody

Research Area

Cancer Biology

Images & Validation

• Tested Applications: ELISA, IF, KO/KD Validated, WB
• Dilution Range: ELISA: 1:10,000 - 1:40,000, IF: 1:500 - 1:2,000, WB: 1:1,000 - 1:4,000
• Reactivity: Frog, Human, Mouse
• Application Notes:
  Anti-beta Actin Antibody has been tested for use in ELISA, immunofluorescence, and western blot. Specific conditions for reactivity should be optimized by the end user. Beta actin present in fibroblast connective tissue stains very brightly. Beta actin present in neuromuscular junctions also stains. Paraformaldehyde fixation yields brighter staining than formalin or methanol fixation. Expect a band at ~42 kDa in size corresponding to beta actin by western blotting in the appropriate cell lysate or extract.

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Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: beta-Actin Loading Control Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a C-Terminal region near amino acids 350-375 of Human beta Actin.
• Target: ACTB
• Purity: Anti-beta-Actin Loading Control Antibody reacts with Human, Rat, Monkey and Mouse. beta-Actin Loading Control Antibody is expected to cross-react with a wide range of species due to sequence homology. Anti-Beta Actin is affinity-purified antibody is directed against human beta Actin protein. A BLAST analysis was used to suggest that this antibody would react with beta Actin from a wide range of organisms, including most vertebrates and some yeast. Broad reactivity makes this antibody an excellent loading control.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 1.0 mg/ml
• Expiration Date: 12 months from date of receipt.
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

rabbit anti-beta Actin Antibody, Actin Antibody, Loading Control Antibody, beta actin, ß actin, anti-beta actin antibody

Quick Database Links

Gene Symbol

ACTB

UniProt

P60709

RefSeq (Protein):NP_001092.1