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Beta-2 Microglobulin Antibody

Catalog Number: orb749752

DispatchUsually dispatched within 5-10 working days
$ 410.00
Catalog Numberorb749752
CategoryAntibodies
DescriptionBeta-2 microglobulin is a 12KDa protein with a pI of 5.6. Serum beta2 microglobulin levels are a reflection of cell turnover. Levels rise with fever, inflammation, and infection. Increased serum levels are also seen in B-cell malignancies and in renal failure and may indicate a worse prognosis for patients with early-stage Hodgkin's lymphoma. In urine, increased levels are seen in proximal renal tubular disease as well as renal transplant rejection. Beta-2 microglobulin levels can rise either because its rate of synthesis has increased (e.g. in AIDS, malignant monoclonal plasma cell dyscrasia, solid tumours and autoimmune disease) or because of impaired renal filtration (e.g. due to renal insufficiency, graft rejection or nephrotoxicity induced by post-transplantation immunosuppressive therapy).
Species/HostMouse
ClonalityMonoclonal
Clone NumberB2M/1118
Tested applicationsFACS, IF, IHC-P, WB
ReactivityHuman
IsotypeMouse IgG1, kappa
ImmunogenFull length recombinant human B2M protein was used as the immunogen for the Beta-2 Microglobulin antibody.
Antibody TypePrimary Antibody
Dilution rangeImmunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT,Western blot: 1-2ug/ml,Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml
PurityProtein G affinity chromatography
ConjugationUnconjugated
Formula0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Hazard InformationThis Beta-2 Microglobulin antibody is available for research use only.
UniProt IDP61769
StorageStore the Beta-2 Microglobulin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
Buffer/Preservatives0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
NoteFor research use only
Application notesOptimal dilution of the Beta-2 Microglobulin antibody should be determined by the researcher.1. Staining of formalin/paraffin tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Expiration Date12 months from date of receipt.
Beta-2 Microglobulin Antibody

IHC: Formalin-fixed, paraffin-embedded human endometrial carcinoma stained with Beta-2-Microglobulin antibody (clone B2M/1118).

Beta-2 Microglobulin Antibody

IHC: Formalin-fixed, paraffin-embedded human cervical carcinoma stained with Beta-2-Microglobulin antibody (clone B2M/1118).

Beta-2 Microglobulin Antibody

IHC: Formalin-fixed, paraffin-embedded human cervical carcinoma stained with Beta-2-Microglobulin antibody (clone B2M/1118).

Beta-2 Microglobulin Antibody

Immunofluorescent staining of permeabilized human HeLa cells with Beta-2 Microglobulin antibody (clone B2M/1118).

Beta-2 Microglobulin Antibody

Western blot testing of human THP-1 and Raji cell lysate with Beta-2 Microglobulin antibody. Expected molecular weight: 12-14 kDa.

Beta-2 Microglobulin Antibody

Flow cytometry testing of permeabilized human HeLa cells with Beta-2 Microglobulin antibody (clone B2M/1118); Red = isotype control, Blue = Beta-2 Microglobulin antibody.

Beta-2 Microglobulin Antibody

SDS-PAGE analysis of purified, BSA-free Beta-2 Microglobulin antibody (clone B2M/1118) as confirmation of integrity and purity.

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