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Catalog Number | orb1273668 |
---|---|
Category | Antibodies |
Description | BACE1 Antibody [3C1C3] |
Species/Host | Mouse |
Clonality | Monoclonal |
Clone Number | 3C1C3 |
Tested applications | ELISA, FC, ICC, WB |
Reactivity | Human, Mouse, Rat |
Isotype | IgG1 |
Immunogen | Rurified recombinant fragment of human BACE1 (AA: 112-324) expressed in E. Coli. |
Antibody Type | Primary Antibody |
Concentration | batch dependent |
Conjugation | Unconjugated |
Target | BACE1 |
UniProt ID | P56817 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | Purified antibody in PBS with 0.05% sodium azide. |
Alternative names | BACE Antibody: ASP2, BACE, HSPC104, KIAA1149, Beta Read more... |
Note | For research use only |
Application notes | ELISA: 1/10000Western Blot: 1/500 - 1/2000ICC: 1/200 - 1/1000Flow Cytometry: 1/200 - 1/400 |
Expiration Date | 12 months from date of receipt. |
Control Antigen (100 ng); Purple line: Antigen(10 ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Western blot analysis using BACE1 mAb against human BACE1 recombinant protein. (Expected MW is 49.9 kDa)
Western blot analysis using BACE1 mAb against HEK293 (1) and BACE1 -hIgGFc transfected HEK293 (2) cell lysate.
Western blot analysis using BACE1 mouse mAb against Hela (1), SK-N-SH (2), HepG2 (3), C6 (4), PC-12 (5), PANC-1 (6), NIH/3T3 (7) cell lysate.
Flow cytometric analysis of Hela cells using BACE1 mouse mAb (green) and negative control (red).
Immunofluorescence analysis of MCF-7 cells using BACE1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. Secondary antibody from Fisher.