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BACE1 Rabbit Polyclonal Antibody

Catalog Number: orb10164

DispatchUsually dispatched within 5-10 working days
$ 210.00
Catalog Numberorb10164
CategoryAntibodies
DescriptionBACE1 Rabbit Polyclonal Antibody
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsICC, IF, IHC-Fr, IHC-P, WB
Predicted ReactivityCanine, Gallus, Guinea pig, Porcine, Rabbit
ReactivityHuman, Mouse, Rat
IsotypeIgG
ImmunogenKLH conjugated synthetic peptide derived from human BACE1 (401-501/501aa)
Antibody TypePrimary Antibody
Concentration1mg/ml
Dilution rangeWB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:100, IF=1:100-500
Form/AppearanceLiquid
ConjugationUnconjugated
MW51 kDa
TargetBACE1
UniProt IDP56817
RRIDAB_10752831
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Alternative namesAPP beta secretase; Asp 2; ASP2; Aspartyl protease
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Background_CLIENT_OVERRIDE_
NoteFor research use only
Expiration Date12 months from date of receipt.
BACE1 Rabbit Polyclonal Antibody

Hela cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (BACE1) polyclonal Antibody, Unconjugated (orb10164) 1:100, 90 minutes at 37°C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

BACE1 Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (mouse pancreas), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (BACE1) Polyclonal Antibody, Unconjugated (orb10164) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

BACE1 Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (rat brain tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (BACE1) Polyclonal Antibody, Unconjugated (orb10164) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.

BACE1 Rabbit Polyclonal Antibody

Sample: Lane 1: SH-SY5Y (Human) Cell Lysate at 30 ug, Lane 2: Huvec (Human) Cell Lysate at 30 ug, Primary: Anti-BACE1 (orb10164) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 60-70 kD, Observed band size: 68 kD.

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