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Catalog Number | orb10164 |
---|---|
Category | Antibodies |
Description | BACE1 Rabbit Polyclonal Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ICC, IF, IHC-Fr, IHC-P, WB |
Predicted Reactivity | Canine, Gallus, Guinea pig, Porcine, Rabbit |
Reactivity | Human, Mouse, Rat |
Isotype | IgG |
Immunogen | KLH conjugated synthetic peptide derived from human BACE1 (401-501/501aa) |
Antibody Type | Primary Antibody |
Concentration | 1mg/ml |
Dilution range | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:100, IF=1:100-500 |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | 51 kDa |
Target | BACE1 |
UniProt ID | P56817 |
RRID | AB_10752831 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
Alternative names | APP beta secretase; Asp 2; ASP2; Aspartyl protease Read more... |
Background | _CLIENT_OVERRIDE_ |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Hela cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (BACE1) polyclonal Antibody, Unconjugated (orb10164) 1:100, 90 minutes at 37°C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.
Paraformaldehyde-fixed, paraffin embedded (mouse pancreas), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (BACE1) Polyclonal Antibody, Unconjugated (orb10164) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat brain tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (BACE1) Polyclonal Antibody, Unconjugated (orb10164) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.
Sample: Lane 1: SH-SY5Y (Human) Cell Lysate at 30 ug, Lane 2: Huvec (Human) Cell Lysate at 30 ug, Primary: Anti-BACE1 (orb10164) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 60-70 kD, Observed band size: 68 kD.
IF, IHC, WB | |
Bovine, Canine, Equine, Guinea pig, Rabbit, Rat, Zebrafish | |
Human, Mouse | |
Rabbit | |
Polyclonal | |
Unconjugated |
WB | |
Canine, Monkey, Mouse, Rat | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |