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ATG5 Antibody
Description
Research Area
Images & Validation
−| Tested Applications | FC, ICC, WB |
|---|---|
| Dilution Range | WB: 1/500 - 1/2000; IF/ICC: 1/200 - 1/1000; FCM: 1/200 - 1/400; ELISA: 1/10000 |
| Reactivity | Human |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | Mouse IgG2a |
| Clone No. | 8E8G6 |
| Immunogen | Synthesized peptide of human ATG5 (AA: MTDDKDVLRDVWFGRIc). |
| Target | Disease |
| Molecular Weight | 32.4kDa |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Purified antibody in PBS with 0.05% sodium azide |
| Concentration | Approximately 1mg/ml (varies from batch to batch). Please inquire for precise concentration. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
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Line graph illustrates about the Ag-Ab reactions using different concentrations of antigen and serial dilutions of ATG5 antibody

Flow cytometric analysis of Hela cells lysates using ATG5 antibody

Western blot analysis of Hela (Lane 1) K562 (Lane 2) cell lysate using ATG5 antibody
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Protocol Information
ATG5 Antibody (orb197763)
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