ATG4B Antibody

SKU: orb1933480

Description

Affinity Purified Rabbit Polyclonal Antibody (Pab)

Research Area

Cell Biology

Images & Validation

• Tested Applications: IHC-P, WB
• Dilution Range: WB - 1:1000, IHC-P - 1:10-50
• Reactivity: Human
• Predicted Reactivity: Mouse, Rat, Zebrafish

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: BTD33065
• Immunogen: This ATG4B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 258-284 amino acids from human ATG4B. Antigen Region: 258-284 aa.
• Target: ATG4B {ECO:0000303|PubMed:15187094, ECO:0000312|HGNC:HGNC:20790}
• Molecular Weight: 44294 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Cysteine protease ATG4B, 3422-, AUT-like 1 cysteine endopeptidase, Autophagin-1, Autophagy-related cysteine endopeptidase 1, Autophagy-related protein 4 homolog B, hAPG4B, ATG4B, APG4B, AUTL1, KIAA0943

Western blot analysis of APG4B (arrow) using purified Pab. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the APG4B gene (Lane 2).

Formalin-fixed and paraffin-embedded human skeletal muscle reacted with Autophagy APG4B Antibody (E273), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Quick Database Links

Gene Symbol

ATG4B {ECO:0000303|PubMed:15187094, ECO:0000312|HGNC:HGNC:20790}

UniProt

Q9Y4P1

RefSeq (Protein):NP_037457.3, NP_847896.1