ATG4B Antibody (N-term)

SKU: orb33345

Description

Purified rabbit polyclonal antibody.

Research Area

Cell Biology

Images & Validation

• Tested Applications: IHC-P, WB
• Dilution Range: WB: 1:1000, IHC-P: 1:50-100
• Reactivity: Human, Mouse

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: BTD9618
• Immunogen: Synthetic Peptide
• Target: ATG4B
• Molecular Weight: 44294
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Anti-APG4B antibody, anti-AUTL1 antibody, anti-MGC1353 antibody, anti-APG4 autophagy 4 homolog B antibody, anti-AUT-like 1 cysteine endopeptidase antibody, anti-autophagin-1 antibody, anti-autophagy-related cysteine endopeptidase 1 antibody, anti-autophagy-related protein 4 homolog B antibody, anti-cysteine protease ATG4B antibody, anti-hAPG4B antibody

The anti-APG4B Pab is used in Western blot to detect APG4B in CEM tissue lysate

APG4B Antibody (R31) western blot analysis in mouse cerebellum tissue lysates (35 ug/lane). This demonstrates the APG4B antibody detected the APG4B protein (arrow).

Western blot analysis of anti-hAPG4B-R31 Pab in 293 cell line lysates transiently transfected with the ATG4B gene (2 ug/lane). hAPG4B-R31 (arrow) was detected using the purified Pab.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Quick Database Links

Gene Symbol

ATG4B

UniProt

Q9Y4P1

RefSeq (Protein):NP_037457.3, NP_847896.1

Yang, Fan et al. HMGA2 plays an important role in Cr (VI)-induced autophagy Int J Cancer, 141, 986-997 (2017)

PubMed: 28510366