Phospho-APG4B (Ser34) Rabbit Polyclonal Antibody

SKU: orb526537

Description

Phospho-APG4B (Ser34) Rabbit Polyclonal Antibody

Research Area

Cell Biology

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P, WB
• Dilution Range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Reactivity: Mouse, Rat
• Predicted Reactivity: Human, Porcine

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated Synthesised phosphopeptide derived from human APG4B around the phosphorylation site of Ser34 KY(p-S)IF
• Target: ATG4B
• Molecular Weight: 44 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

ATG4B | APG4B (p-S34); p-APG4B; phospho-APG4B; APG4B; AUTL1; HsAPG4B; 2510009N07Rik; Atg4bl; MmAPG4B; ATG4B_HUMAN; ATG4B; AUT-like 1 cysteine endopeptidase; Autophagy-related cysteine endopeptidase 1 (Autophagin-1); Autophagy-related protein 4 homolog B (HsAPG4B | hAPG4B); 3.4.22.-; KIAA0943; ATG4B_MOUSE; Autophagy-related protein 4 homolog B (MmAPG4B); MLP3B_RAT; Map1lc3b; Autophagy-related protein LC3 B; Autophagy-related ubiquitin-like modifier LC3 B; MAP1 light chain 3-like protein 2; Microtubule-associated proteins 1A/1B light chain 3B (MAP1A/MAP1B LC3 B | MAP1A/MAP1B light chain 3 B); Map1alc3; Map1lc3;

Paraformaldehyde-fixed, paraffin embedded (Rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (phospho-APG4B (Ser34)) Polyclonal Antibody, Unconjugated (orb526537) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Lane 1: Mouse Muscle tissue lysates, Lane 2: Mouse Cerebrum tissue lysates, Lane 3: Mouse Heart tissue lysates, Primary: Anti-phospho-APG4B (Ser34) (orb526537) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 44 kDa, Observed band size: 50 kDa.

Quick Database Links

Gene Symbol

ATG4B

UniProt

Q9Y4P1