ATG4B Antibody (C-term)

• Catalog Number: orb1933481
• Category: Antibodies
• Description: ATG4B Antibody (C-term)
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Mouse
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• UniProt ID: Q9Y4P1
• MW: 44294 Da
• Tested applications: IF, IHC-P, WB
• Dilution range: IF - 1:100, WB - 1:1000, IHC-P - 1:100-500
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: APG4B, AUTL1, KIAA0943
• Research Area: Cancer Biology, Cardiovascular Research, Cell Biol
• Note: For research use only

Western blot analysis of anti-APG4B Pab in Hela cell lysate. APG4B (arrow) was detected using the purified Pab.

Western blot analysis of anti-hAPG4B-373 Pab in 293 cell line lysates transiently transfected with the ATG4B gene (2 ug/lane). hAPG4B-373 (arrow) was detected using the purified Pab.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Fluorescent image of U251 cells stained with ATG4B (C-term) antibody. U251 cells were treated with Chloroquine (50 μM, 16 h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with ATG4B (C-term) primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1 h). Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/ml, 5 min). ATG4B immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.