ATG4B Antibody

SKU: orb1240182

Description

ATG4B Antibody

Research Area

Cell Biology

Images & Validation

• Tested Applications: ELISA, IF, IHC-P, WB
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Bovine, Gallus
• Application Notes:
  ATG4B antibody can be used for detection of ATG4B by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: ATG4B antibody was raised against a 17 amino acid peptide near the carboxy terminus of human ATG4B.The immunogen is located within the last 50 amino acids of ATG4B.
• Target: ATG4B
• Molecular Weight: Predicted: 43 kDa Observed: 46 kDa
• Purification: ATG4B antibody is affinity chromatography purified via peptide column.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: ATG4B antibody is supplied in PBS containing 0.02% sodium azide.
• Concentration: 1 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Autophagy related 4B cysteine peptidase, APG4B, APG4-B, AUTL1

Western blot analysis of ATG4B in (A) Ramos and (B) Jurkat cell lysate with ATG4B antibody at 1 µg/mL.

Immunohistochemistry of ATG4B in human spleen tissue with ATG4B antibody at 5 µg/ml.

Immunofluorescence of ATG4B in human spleen tissue with ATG4B antibody at 20 µg/ml.

Quick Database Links

Gene Symbol

ATG4B

UniProt

Q9Y4P1

RefSeq (Protein):NP_037457