Anti-TNF alpha Antibody

• Catalog Number: orb334806
• Category: Antibodies
• Description: Rabbit polyclonal antibody to TNF alpha
• Target: TNF
• Clonality: Polyclonal
• Species/Host: Rabbit
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Immunogen: Recombinant protein corresponding to human TNF alpha.
• UniProt ID: P06804, P01375, P16599
• Tested applications: IH, WB
• Dilution range: WB: 1:1000-1:2000, IHC-P: 1:50-1:200
• Antibody Type: Primary Antibody
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti Serotransferrin antibody, anti Transferrin an
• Note: For research use only
• Entrez: 21926, 24835, 7124

Western blot analysis of TNF alpha expression in HepG2 LPS-treated (A) whole cell lysates. (Predicted band size: 25 kD; Observed band size: 17; 25 kD)

Immunohistochemical analysis of TNF alpha staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.