Anti-SMAD5 Antibody

• Catalog Number: orb705630
• Category: Antibodies
• Description: Rabbit polyclonal antibody to SMAD5
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IH, WB
• Reactivity: Human, Mouse, Rat
• Immunogen: Recombinant fusion protein of human SMAD5. The exact sequence is proprietary.
• Antibody Type: Primary Antibody
• Dilution range: WB: 1:500-1:1000, IF: 1:50-1:100
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: SMAD5
• Entrez: 17129, 4090, 59328
• UniProt ID: P97454, Q9R1V3, Q99717
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: Anti-MADH5 antibody, anti-Mothers against decapent
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of SMAD5 expression in H460 (A) whole cell lysates. (Predicted band size: 52 kD; Observed band size: 52 kD)

Immunohistochemical analysis of SMAD5 staining in human colon formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.