SMAD1 + SMAD5 Rabbit Polyclonal Antibody

SKU: orb100675

Description

SMAD1 + SMAD5 Rabbit Polyclonal Antibody

Research Area

Neuroscience

Images & Validation

• Tested Applications: ICC, IF, IHC-Fr, IHC-P, WB
• Dilution Range: WB=1:500-1000, IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:100-500, IF=1:100-500
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Bovine, Canine, Equine, Gallus, Rabbit

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human SMAD5 (361-465/465aa)
• Target: SMAD5
• Molecular Weight: 51 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

DWFC; JV5-1; MADH5; 1110051M15Rik; Dwf-C; MusMLP; SMAD5_HUMAN; SMAD5; MAD homolog 5; Mothers against DPP homolog 5; SMAD family member 5 (SMAD 5 | Smad5 | hSmad5); SMAD5_MOUSE; Dwarfin-C (Dwf-C); SMAD family member 5 (SMAD 5 | Smad5 | mSmad5); Msmad5; SMAD5_RAT; SMAD family member 5 (SMAD 5 | Smad5);

Hela cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (SMAD1 + SMAD5) polyclonal Antibody, Unconjugated (orb100675) 1:100, 90 minutes at 37°C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (SMAD1 + SMAD5) Polyclonal Antibody, Unconjugated (orb100675) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Lane 1: Mouse Cerebrum tissue lysates, Lane 2: Rat Cerebrum tissue lysates, Primary: Anti-SMAD1 + SMAD5 (orb100675) at 1/200 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 51 kDa, Observed band size: 55 kDa.

Quick Database Links

Gene Symbol

SMAD5

UniProt

Q99717