Phospho-Smad1/5 (Ser463 + Ser465) Rabbit Polyclonal Antibody

• Catalog Number: orb6977
• Category: Antibodies
• Description: Phospho-Smad1/5 (Ser463 + Ser465) Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Bovine, Canine, Equine, Gallus
• Reactivity: Human, Mouse, Porcine, Rat
• Isotype: IgG
• Immunogen: KLH conjugated Synthesised phosphopeptide derived from human Smad1/5 around the phosphorylation site of Ser463/465 IS(p-S)V(p-S)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1μg/Test
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 52 kDa
• Target: SMAD1
• UniProt ID: Q15797
• RRID: AB_10931262
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: Smad1(phospho S463/465); Smad1(phospho Ser463/465)
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Cerebrum (Mouse) Lysate at 40 ug, Stomach (Mouse) Lysate at 40 ug, Primary: Anti-Phospho-Smad1/5 (Ser463 + Ser465) (orb6977) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 52 kD, Observed band size: 62 kD.

Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Phospho-Smad1 5 (Ser463 + Ser465)) Polyclonal Antibody, Unconjugated (orb6977) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.

Paraformaldehyde-fixed, paraffin embedded (mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Phospho-Smad1-5 (Ser463+ Ser465)) Polyclonal Antibody, Unconjugated (orb6977) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Lane 1: Cerebrum (Mouse) Lysate at 40 ug, Lane 2: Heart (Mouse) Lysate at 40 ug, Lane 3: Testis (Mouse) Lysate at 40 ug, Lane 4: Skin (Mouse) Lysate at 40 ug, Lane 5: Kidney (Mouse) Lysate at 40 ug, Lane 6: Cerebrum (Rat) Lysate at 40 ug, Lane 7: Testis (Rat) Lysate at 40 ug, Lane 8: Skin (Rat) Lysate at 40 ug, Lane 9: Kidney (Rat) Lysate at 40 ug, Lane 10: Huvec (Human) Cell Lysate at 30 ug, Lane 11: A549 (Human) Cell Lysate at 30 ug, Lane 12: Hela (Human) Cell Lysate at 30 ug, Lane 13: HT1080 (Human) Cell Lysate at 30 ug, Lane 14: A431 (Human) Cell Lysate at 30 ug, Primary: Anti-Phospho-Smad3 (Ser463 + Ser465) (orb6977) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 52 kD, Observed band size: 54 kD.