Phospho-Smad1/5 (Ser463 + Ser465) Rabbit Polyclonal Antibody

SKU: orb6977

Description

Phospho-Smad1/5 (Ser463 + Ser465) Rabbit Polyclonal Antibody

Research Area

Neuroscience

Images & Validation

• Tested Applications: FC, IF, IHC-Fr, IHC-P, WB
• Dilution Range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1μg/Test
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Bovine, Canine, Equine, Gallus, Porcine

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated Synthesised phosphopeptide derived from human Smad1/5 around the phosphorylation site of Ser463/465 IS(p-S)V(p-S)
• Target: SMAD1
• Molecular Weight: 52 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

SMAD1 & SMAD5 (p-S463/S465); p-SMAD1 & SMAD5; phospho-SMAD1 & SMAD5; BSP-1; BSP1; JV4-1; JV41; MADH1; MADR1; Mad1; Mlp1; MusMLP; dwf-A; mMad1; SMAD1_HUMAN; SMAD1; MAD homolog 1; Mothers against DPP homolog 1; Mad-related protein 1; SMAD family member 1 (SMAD 1 | Smad1 | hSMAD1); Transforming growth factor-beta-signaling protein 1 (BSP-1); SMAD1_MOUSE; Dwarfin-A (Dwf-A); Mothers-against-DPP-related 1 (Mad-related protein 1 | mMad1); SMAD family member 1 (SMAD 1 | Smad1); SMAD1_RAT;

Cerebrum (Mouse) Lysate at 40 ug, Stomach (Mouse) Lysate at 40 ug, Primary: Anti-Phospho-Smad1/5 (Ser463 + Ser465) (orb6977) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 52 kD, Observed band size: 62 kD.

Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Phospho-Smad1 5 (Ser463 + Ser465)) Polyclonal Antibody, Unconjugated (orb6977) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.

Paraformaldehyde-fixed, paraffin embedded (mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Phospho-Smad1-5 (Ser463+ Ser465)) Polyclonal Antibody, Unconjugated (orb6977) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Lane 1: Cerebrum (Mouse) Lysate at 40 ug, Lane 2: Heart (Mouse) Lysate at 40 ug, Lane 3: Testis (Mouse) Lysate at 40 ug, Lane 4: Skin (Mouse) Lysate at 40 ug, Lane 5: Kidney (Mouse) Lysate at 40 ug, Lane 6: Cerebrum (Rat) Lysate at 40 ug, Lane 7: Testis (Rat) Lysate at 40 ug, Lane 8: Skin (Rat) Lysate at 40 ug, Lane 9: Kidney (Rat) Lysate at 40 ug, Lane 10: Huvec (Human) Cell Lysate at 30 ug, Lane 11: A549 (Human) Cell Lysate at 30 ug, Lane 12: Hela (Human) Cell Lysate at 30 ug, Lane 13: HT1080 (Human) Cell Lysate at 30 ug, Lane 14: A431 (Human) Cell Lysate at 30 ug, Primary: Anti-Phospho-Smad3 (Ser463 + Ser465) (orb6977) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 52 kD, Observed band size: 54 kD.

Quick Database Links

Gene Symbol

SMAD1

UniProt

Q15797